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Type: Journal article
Title: Targeting GRP78 to enhance melanoma cell death
Author: Martin, S.
Hill, D.
Paton, J.
Paton, A.
Birch-Machin, M.
Lovat, P.
Redfern, C.
Citation: Pigment Cell & Melanoma Research, 2010; 23(5):675-682
Publisher: Wiley-Blackwell Publishing
Issue Date: 2010
ISSN: 1755-1471
Statement of
Shaun Martin, David S. Hill, James C. Paton, Adrienne W. Paton, Mark A. Birch-Machin, Penny E. Lovat, Chris P.F. Redfern
Abstract: Targeting endoplasmic reticulum stress-induced apoptosis may offer an alternative therapeutic strategy for metastatic melanoma. Fenretinide and bortezomib induce apoptosis of melanoma cells but their efficacy may be hindered by the unfolded protein response, which promotes survival by ameliorating endoplasmic reticulum stress. The aim of this study was to test the hypothesis that inhibition of GRP78, a vital unfolded protein response mediator, increases cell death in combination with endoplasmic reticulum stress-inducing agents. Down-regulation of GRP78 by small-interfering RNA increased fenretinide- or bortezomib-induced apoptosis. Treatment of cells with a GRP78-specific subtilase toxin produced a synergistic enhancement with fenretinide or bortezomib. These data suggest that combining endoplasmic reticulum stress-inducing agents with strategies to down-regulate GRP78, or other components of the unfolded protein response, may represent a novel therapeutic approach for metastatic melanoma.
Keywords: melanoma; glucose-regulated protein 78; endoplasmic reticulum stress; fenretinide; bortezomib
Rights: © 2010 John Wiley & Sons A/S
RMID: 0020100784
DOI: 10.1111/j.1755-148X.2010.00731.x
Appears in Collections:Molecular and Biomedical Science publications

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