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Type: Journal article
Title: Whole-body heat exposure induces membrane changes in spermatozoa from the cauda epididymidis of laboratory mice
Author: Wechalekar, H.
Setchell, B.
Peirce, E.
Ricci, M.
Leigh, C.
Breed, W.
Citation: Asian Journal of Andrology, 2010; 12(4):591-598
Publisher: Blackwell Publishing
Issue Date: 2010
ISSN: 1008-682X
Statement of
Harsha Wechalekar, Brian P. Setchell, Eleanor J. Peirce, Mario Ricci, Chris Leigh and William G. Breed
Abstract: This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of laboratory mice was investigated. C57BL/6 mice (n = 7) were housed in a microclimate chamber at 37ºC–38ºC for 8 h per day for three consecutive days, while control mice (n = 7) were kept at 23ºC–24ºC. Cauda epididymal spermatozoa were obtained 16 h after the last heat treatment. The results showed that sperm numbers were similar in the two groups (P = 0.23), but after heat treatment, a significant reduction in the percentage of motile sperm was present (P < 0.0001). Membrane changes of the spermatozoa were investigated by staining with phycoerythrin (PE)- conjugated Annexin V, which detects exteriorization of phosphotidylserine from the inner to the outer leaflet of the sperm plasma membrane, and 7-aminoactinomycin D (7-AAD), which binds to the sperm nucleus when the plasma membrane is damaged. The percentage of spermatozoa showing positive staining with Annexin V–PE or 7-AAD or both, was significantly higher (P < 0.05) in heat-exposed mice compared with controls. These results show that whole-body heat exposure to 37ºC–38ºC induces membrane changes in the epididymal spermatozoa of mice, which may lead to apoptosis.
Keywords: laboratory mice
sperm membrane damage
whole-body heat
Rights: © 2010 AJA, SIMM & SJTU All rights reserved
DOI: 10.1038/aja.2010.41
Appears in Collections:Anatomical Sciences publications
Aurora harvest
Environment Institute publications

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