Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Whole-body heat exposure induces membrane changes in spermatozoa from the cauda epididymidis of laboratory mice|
|Citation:||Asian Journal of Andrology, 2010; 12(4):591-598|
|Harsha Wechalekar, Brian P. Setchell, Eleanor J. Peirce, Mario Ricci, Chris Leigh and William G. Breed|
|Abstract:||This study was carried out to determine if exposure to hot environmental temperatures had a direct, detrimental effect on sperm quality. For this the effect of whole-body heat exposure on epididymal spermatozoa of laboratory mice was investigated. C57BL/6 mice (n = 7) were housed in a microclimate chamber at 37ºC–38ºC for 8 h per day for three consecutive days, while control mice (n = 7) were kept at 23ºC–24ºC. Cauda epididymal spermatozoa were obtained 16 h after the last heat treatment. The results showed that sperm numbers were similar in the two groups (P = 0.23), but after heat treatment, a significant reduction in the percentage of motile sperm was present (P < 0.0001). Membrane changes of the spermatozoa were investigated by staining with phycoerythrin (PE)- conjugated Annexin V, which detects exteriorization of phosphotidylserine from the inner to the outer leaflet of the sperm plasma membrane, and 7-aminoactinomycin D (7-AAD), which binds to the sperm nucleus when the plasma membrane is damaged. The percentage of spermatozoa showing positive staining with Annexin V–PE or 7-AAD or both, was significantly higher (P < 0.05) in heat-exposed mice compared with controls. These results show that whole-body heat exposure to 37ºC–38ºC induces membrane changes in the epididymal spermatozoa of mice, which may lead to apoptosis.|
|Keywords:||laboratory mice; sperm membrane damage; whole-body heat|
|Rights:||© 2010 AJA, SIMM & SJTU All rights reserved|
|Appears in Collections:||Anatomical Sciences publications|
Environment Institute publications
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.