Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/62812
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Type: Journal article
Title: Macrophages define dermal lymphatic vessel calibre during development by regulating lymphatic endothelial cell proliferation
Author: Gordon, E.
Rao, S.
Pollard, J.
Nutt, S.
Lang, R.
Harvey, N.
Citation: Development, 2010; 137(22):3899-3910
Publisher: Company of Biologists Ltd
Issue Date: 2010
ISSN: 0950-1991
1477-9129
Statement of
Responsibility: 
Emma J. Gordon, Sujata Rao, Jeffrey W. Pollard, Stephen L. Nutt, Richard A. Lang and Natasha L. Harvey
Abstract: Macrophages have been suggested to stimulate neo-lymphangiogenesis in settings of inflammation via two potential mechanisms: (1) acting as a source of lymphatic endothelial progenitor cells via the ability to transdifferentiate into lymphatic endothelial cells and be incorporated into growing lymphatic vessels; and (2) providing a crucial source of pro-lymphangiogenic growth factors and proteases. We set out to establish whether cells of the myeloid lineage are important for development of the lymphatic vasculature through either of these mechanisms. Here, we provide lineage tracing evidence to demonstrate that lymphatic endothelial cells arise independently of the myeloid lineage during both embryogenesis and tumour-stimulated lymphangiogenesis in the mouse, thus excluding macrophages as a source of lymphatic endothelial progenitor cells in these settings. In addition, we demonstrate that the dermal lymphatic vasculature of PU.1–/– and Csf1r–/– macrophage-deficient mouse embryos is hyperplastic owing to elevated lymphatic endothelial cell proliferation, suggesting that cells of the myeloid lineage provide signals that act to restrain lymphatic vessel calibre in the skin during development. In contrast to what has been demonstrated in settings of inflammation, macrophages do not comprise the principal source of pro-lymphangiogenic growth factors, including VEGFC and VEGFD, in the embryonic dermal microenvironment, illustrating that the sources of patterning and proliferative signals driving embryonic and disease-stimulated lymphangiogenesis are likely to be distinct.
Keywords: Lymphangiogenesis; Lymphatic vessels; Macrophages; Vascular development; Mouse
Rights: © 2010. Published by The Company of Biologists Ltd.
RMID: 0020101287
DOI: 10.1242/dev.050021
Grant ID: http://purl.org/au-research/grants/nhmrc/453493
Appears in Collections:Medicine publications

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