Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66477
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Type: | Journal article |
Title: | Comparison of blood sirolimus, tacrolimus and everolimus concentrations measured by LC-MS/MS, HPLC-UV and immunoassay methods |
Author: | Sallustio, B. Noll, B. Morris, R. |
Citation: | Clinical Biochemistry, 2011; 44(2-3):231-236 |
Publisher: | Pergamon-Elsevier Science Ltd |
Issue Date: | 2011 |
ISSN: | 0009-9120 1873-2933 |
Statement of Responsibility: | Benedetta C. Sallustio, Benjamin D. Noll, Raymond G. Morris |
Abstract: | <h4>Objectives</h4>An LC-MS/MS method was developed for simultaneous quantitation of tacrolimus, sirolimus and everolimus in whole blood, and compared to HPLC-UV and immunoassay methods.<h4>Design and methods</h4>Blood (0.1mL) was analysed following solid-phase extraction and chromatographic resolution using a C18 column (45°C) and mobile phase of methanol/40mM ammonium acetate/glacial acetic acid (83/17/0.1) at 200μL/min, with positive electrospray ionisation and multiple reaction monitoring.<h4>Results</h4>Intra- and inter-day imprecision and inaccuracy were ≤12.2% over a 1.5-40μg/L calibration range. An external quality assurance programme confirmed acceptable inaccuracy and imprecision of the LC-MS/MS method, but highlighted problems with immunoassay quantitation, particularly for everolimus, showing a >30% bias in FPIA everolimus concentrations measured in pooled patient samples versus spiked drug-free whole blood.<h4>Conclusions</h4>LC-MS/MS provides significant accuracy and precision advantages compared to HPLC and immunoassays. Discrepancies in everolimus concentrations measured by the Seradyn FPIA immunoassay require further investigation. |
Keywords: | LC-MS/MS Immunoassays Sirolimus Tacrolimus Everolimus |
Rights: | Crown copyright © 2010 Published by Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.clinbiochem.2010.10.005 |
Published version: | http://dx.doi.org/10.1016/j.clinbiochem.2010.10.005 |
Appears in Collections: | Aurora harvest 5 Pharmacology publications |
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hdl_66477.pdf | Accepted version | 1.26 MB | Adobe PDF | View/Open |
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