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https://hdl.handle.net/2440/66710
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Type: | Journal article |
Title: | Optimization of the Production of Biosurfactant From Iranian Indigenous Bacteria for the Reduction of Surface Tension and Enhanced Oil Recovery |
Author: | Amani, H. Haghighi, M. Sarrafzadeh, M. Mehrnia, M. Shahmirzaee, F. |
Citation: | Petroleum Science and Technology, 2011; 29(3):301-311 |
Publisher: | Marcel Dekker Inc |
Issue Date: | 2011 |
ISSN: | 1091-6466 1532-2459 |
Statement of Responsibility: | H. Amani, M. Haghighi, M. H. Sarrafzadeh, M. R. Mehrnia, and F. Shahmirzaee |
Abstract: | The optimum conditions for biosurfactant production by Iran's isolates were examined. The Taguchi method was used to identify nutritional requirements in the medium using four parameters; that is, carbon source, nitrogen, phosphorous, and salt concentrations. The use of whey, oil, and sucrose as carbon sources; NaCl as salt source; (Na2HPO4, NaH2PO 4) as phosphorous source; and (NH4)2SO 4 as nitrogen source was examined to determine bacteria optimum conditions. According to the Taguchi method using the sucrose source, the optimal conditions for Bacillus subtilis were 50 g/L NaCl, 13.53 g/L (Na 2HPO4, NaH2PO4), and 1 g/L (NH 4)2SO4; for Bacillus cereus they were 25 g/L NaCl, 13.53 g/L (Na2HPO4, NaH2PO4), and 1 g/L (NH4)2SO4; and for Pseudomonas putida they were 25 g/L NaCl, 13.53 g/L (Na2HPO4, NaH 2PO4), and 1 g/L (NH4)2SO 4. Oil displacement experiments in the micromodel at optimum conditions showed around 25% recovery of residual oil with added supernatant of Bacillus subtilis. Copyright © Taylor & Francis Group, LLC. |
Keywords: | Bacillus cereus Bacillus subtilis biosurfactant Pseudomonas putida surface tension Taguchi method |
Rights: | Copyright © Taylor & Francis Group, LLC |
DOI: | 10.1080/10916460903393963 |
Published version: | http://dx.doi.org/10.1080/10916460903393963 |
Appears in Collections: | Aurora harvest Australian School of Petroleum publications |
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