Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/6783
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dc.contributor.authorGronthos, S.-
dc.contributor.authorZannettino, A.-
dc.contributor.authorGraves, S.-
dc.contributor.authorOhta, S.-
dc.contributor.authorHay, S.-
dc.contributor.authorSimmons, P.-
dc.date.issued1999-
dc.identifier.citationJournal of Bone and Mineral Research, 1999; 14(1):47-56-
dc.identifier.issn0884-0431-
dc.identifier.issn1523-4681-
dc.identifier.urihttp://hdl.handle.net/2440/6783-
dc.description.abstractHuman osteoblast-like cells can be readily cultured from explants of trabecular bone, reproducibly expressing the characteristics of cells belonging to the osteoblastic lineage. Dual-color fluorescence-activated cell sorting was employed to develop a model of bone cell development in primary cultures of normal human bone cells (NHBCs) based on the cell surface expression of the stromal precursor cell marker STRO-1 and the osteoblastic marker alkaline phosphatase (ALP). Cells expressing the STRO-1 antigen exclusively (STRO-1+/ALP-), were found to exhibit qualities preosteoblastic in nature both functionally by their reduced ability to form a mineralized bone matrix over time, as measured by calcium release assay, and in the lack of their expression of various bone-related markers including bone sialoprotein, osteopontin, and parathyroid hormone receptor based on reverse trancriptase polymerase chain reaction (PCR) analysis. The majority of the NHBCs which expressed the STRO-1-/ALP+ and STRO-1-/ALP- phenotypes appeared to represent fully differentiated osteoblasts, while the STRO-1+/ALP+ subset represented an intermediate preosteoblastic stage of development. All STRO-1/ALP NHBC subsets were also found to express the DNA-binding transcription factor CBFA-1, confirming that these cultures represent committed osteogenic cells. In addition, our primer sets yielded four distinct alternative splice variants of the expected PCR product for CBFA-1 in each of the STRO-1/ALP subsets, with the exception of the proposed preosteoblastic STRO-1+/ALP- subpopulation. Furthermore, upon re-culture of the four different STRO-1/ALP subsets only the STRO-1+/ALP- subpopulation was able to give rise to all of the four subsets yielding the same proportions of STRO-1/ALP expression as in the original primary cultures. The data presented in this study demonstrate a hierarchy of bone cell development in vitro and facilitate the study of bone cell differentiation and function.-
dc.language.isoen-
dc.publisherWILEY-
dc.source.urihttp://dx.doi.org/10.1359/jbmr.1999.14.1.47-
dc.subjectBone Matrix-
dc.subjectCells, Cultured-
dc.subjectHumans-
dc.subjectAlkaline Phosphatase-
dc.subjectTranscription Factors-
dc.subjectAntibodies, Monoclonal-
dc.subjectAntigens, Surface-
dc.subjectCell Lineage-
dc.subjectBone Development-
dc.subjectOsteogenesis-
dc.subjectBone Density-
dc.subjectPhenotype-
dc.subjectReference Values-
dc.subjectAged-
dc.subjectAged, 80 and over-
dc.subjectMiddle Aged-
dc.subjectBiomarkers-
dc.titleDifferential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cells-
dc.typeJournal article-
dc.identifier.doi10.1359/jbmr.1999.14.1.47-
pubs.publication-statusPublished-
dc.identifier.orcidGronthos, S. [0000-0002-6225-3084]-
dc.identifier.orcidZannettino, A. [0000-0002-6646-6167]-
dc.identifier.orcidGraves, S. [0000-0002-1629-319X]-
Appears in Collections:Aurora harvest
Orthopaedics and Trauma publications

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