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https://hdl.handle.net/2440/68870
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Type: | Journal article |
Title: | The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line |
Author: | Lam, Y. Janovska, A. McAinch, A. Belobrajdic, D. Hatzinikolas, G. Game, P. Wittert, G. |
Citation: | Obesity Research and Clinical Practice, 2011; 5(1):43-54 |
Publisher: | Elsevier BV |
Issue Date: | 2011 |
ISSN: | 1871-403X |
Statement of Responsibility: | Yan Y. Lam, Alena Janovská, Andrew J. McAinch, Damien P. Belobrajdic, George Hatzinikolas, Philip Game, Gary A. Wittert |
Abstract: | <h4>Summary</h4>We aimed to study the depot-specific effect of adipose tissue on insulin sensitivity of skeletal muscle in vitro. Adipose tissue-conditioned medium (CM) was generated from visceral and subcutaneous fat from obese subjects. CM from visceral as compared to subcutaneous fat had higher concentrations of interleukin (IL)-6 (15-fold; P < 0.05) and IL-8 (8-fold; P < 0.05). CM from visceral fat (1:128 dilution) reduced insulin-stimulated glucose uptake in L6 myotubes by 19% (P < 0.05), an effect mediated by a nuclear factor kappa B (NFκB)/mammalian target of rapamycin complex 1 (mTORC1)-dependent pathway and partially reversed by neutralizing IL-6. IL-6 at a concentration comparable to that in CM from visceral fat reduced insulin-stimulated glucose uptake by 53% (P < 0.05), an effect abolished by inhibiting NFκB or mTORC1. We demonstrated the utility of the CM-myotube system and identified IL-6 as a major cytokine mediating visceral fat-induced muscle insulin resistance.: |
Keywords: | Adipokines Skeletal muscle Insulin resistance |
Rights: | Copyright © 2010 Asian Oceanian Association for the Study of Obesity. |
DOI: | 10.1016/j.orcp.2010.12.002 |
Published version: | http://dx.doi.org/10.1016/j.orcp.2010.12.002 |
Appears in Collections: | Aurora harvest Medicine publications |
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