Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/68993
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Type: Journal article
Title: Changes in cardiac troponins with gestational age explain changes in cardiac muscle contractility in the sheep fetus
Author: Posterino, Giuseppe Saverio
Dunn, Stacey Leanne
Botting, Kimberley Jae
Wang, William
Gentili, Sheridan
Morrison, Janna Leigh
Citation: Journal of Applied Physiology, 2011; 111(1):236-243
Publisher: Amer Physiological Soc
Issue Date: 2011
ISSN: 8750-7587
School/Discipline: School of Medical Sciences
Statement of
Responsibility: 
Giuseppe Saverio Posterino, Stacey Leanne Dunn, Kimberley Jae Botting, William Wang, Sheridan Gentili and Janna Leigh Morrison
Abstract: The development of the adult cardiac troponin complex in conjunction with changes in cardiac function and cardiomyocyte binucleation has not been systematically characterized during fetal life in a species where maturation of the cardiomyocytes occurs prenatally as it does in the human. The aim of this study was to correlate the expression of each of the major adult troponin isoforms (T, I, and C) during late gestation (term of 150 days) to changes in both Ca²⁺ sensitivity and maximum Ca²⁺-activated force of the contractile apparatus and the maturation of cardiomyocytes. The percentage of mononucleated cardiomyocytes in the right ventricle decreased with gestational age to 46% by 137–142 days of gestation. The length of binucleated cardiomyocytes did not change with gestational age, but the length of binucleated cardiomyocytes relative to heart weight decreased with gestational age. There was no change in the expression of adult cardiac troponin T with increasing gestation. The contractile apparatus was significantly more sensitive to Ca²⁺ at 90 days compared with either 132 or 139 days of gestation, consistent with an ∼30% increase in the expression of adult cardiac troponin I between 90 and 110 days of gestation. Maximum Ca²⁺-activated force significantly increased from 90 days compared with 130 days consistent with an increase of ∼40% in cardiac troponin C protein expression. These data show that increased adult cardiac troponin I and C protein expression across late gestation is consistent with reduced Ca²⁺ sensitivity and increased maximum Ca²⁺-activated force. Furthermore, changes in cardiac troponin C, not I, protein expression track with the timing of cardiomyocyte binucleation.
Keywords: heart; calcium
Rights: Copyright © 2011 the American Physiological Society
RMID: 0020111128
DOI: 10.1152/japplphysiol.00067.2011
Appears in Collections:Physiology publications

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