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https://hdl.handle.net/2440/69030
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Type: | Journal article |
Title: | Isolation and in vitro characterization of putative porcine embryonic stem cells from cloned embryos treated with Trichostatin A |
Author: | Vassiliev, I. Vassilieva, S. Truong, K. Beebe, L. McIlfatrick, S. Harrison, S. Nottle, M. |
Citation: | Cellular Reprogramming, 2011; 13(3):205-213 |
Publisher: | Mary Ann Liebert, Inc. |
Issue Date: | 2011 |
ISSN: | 2152-4971 2152-4998 |
Statement of Responsibility: | Ivan Vassiliev, Svetlana Vassilieva, Kam P. Truong, Luke F.S. Beebe, Stephen M. McIlfatrick, Sharon J. Harrison, and Mark B. Nottle |
Abstract: | We report here the establishment and characterization of putative porcine embryonic stem cell (ESC) lines derived from somatic cell nuclear transfer embryos (NT-ESCs). These cells had a similar morphology to that described previously by us for ESCs derived from in vitro produced embryos, namely, a polygonal shape, a relatively small (10–15 μm) diameter, a small cytoplasmic/nuclear ratio, a single nucleus with multiple nucleoli and multiple lipid inclusions in the cytoplasm. NT-ESCs could be passaged at least 15 times and vitrified repeatedly without changes in their morphology, karyotype, or Oct-4 and Nanog expression. These cells formed embryoid bodies and could be directed to differentiate in vitro to cell types representative of all three germ layers. Following their injection into blastocysts, these cells preferentially localized in the inner cell mass. In conclusion, we have isolated putative porcine ESCs from cloned embryos that have the potential to be used for a variety of applications including as a model for human therapeutic cloning. |
Keywords: | Cells, Cultured Cell Line Blastocyst Animals Swine Hydroxamic Acids Cell Separation Cloning, Organism Karyotyping Models, Animal Female Embryonic Stem Cells Embryo, Mammalian Histone Deacetylase Inhibitors |
Rights: | © Mary Ann Liebert, Inc. |
DOI: | 10.1089/cell.2010.0102 |
Published version: | http://dx.doi.org/10.1089/cell.2010.0102 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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