Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/69138
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dc.contributor.authorThomas, P.en
dc.contributor.authorHolland, N.en
dc.contributor.authorBolognesi, C.en
dc.contributor.authorKirsch-Volders, M.en
dc.contributor.authorBonassi, S.en
dc.contributor.authorZeiger, E.en
dc.contributor.authorKnasmueller, S.en
dc.contributor.authorFenech, M.en
dc.date.issued2009en
dc.identifier.citationNature Protocols (Print), 2009; 4(6):825-837en
dc.identifier.issn1754-2189en
dc.identifier.issn1750-2799en
dc.identifier.urihttp://hdl.handle.net/2440/69138-
dc.description.abstractThe Buccal Micronucleus Cytome (BMCyt) assay is a minimally invasive method for studying DNA damage, chromosomal instability, cell death and the regenerative potential of human buccal mucosal tissue. This method is increasingly used in molecular epidemiological studies for investigating the impact of nutrition, lifestyle factors, genotoxin exposure and genotype on DNA damage, chromosome malsegregation and cell death. The biomarkers measured in this assay have been associated with increased risk of accelerated ageing, cancer and neurodegenerative diseases. This protocol describes one of the current established methods for buccal cell collection using a small-headed toothbrush, the generation of a single-cell suspension, slide preparation using cytocentrifugation, fixation and staining using Feulgen and Light Green for both bright field and fluorescence microscopic analysis. The scoring criteria for micronuclei and other nuclear anomalies are also described in detail. The protocol in its current form takes approximately 4 h to complete from the time of buccal cell collection to the generation of stained slides for microscopic analysis.en
dc.description.statementofresponsibilityPhilip Thomas, Nina Holland, Claudia Bolognesi, Micheline Kirsch-Volders, Stefano Bonassi, Errol Zeiger, Siegfried Knasmueller & Michael Fenechen
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.rights© 2009 Nature Publishing Groupen
dc.subjectDetection and probing; Imaging; Spectroscopyen
dc.titleBuccal micronucleus cytome assayen
dc.typeJournal articleen
dc.identifier.rmid0020090766en
dc.identifier.doi10.1038/nprot.2009.53en
dc.identifier.pubid38833-
pubs.library.collectionPharmacology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Pharmacology publications

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