Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7006
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Characterization and chromosomal localization of the human A2a adenosine receptor gene - ADORA2A |
Author: | Le, F. Townsend-Nicholson, A. Baker, E. Sutherland, G. Schofield, P. |
Citation: | Biochemical and Biophysical Research Communications, 1996; 223(2):461-467 |
Publisher: | ACADEMIC PRESS INC ELSEVIER SCIENCE |
Issue Date: | 1996 |
ISSN: | 0006-291X 1090-2104 |
Statement of Responsibility: | Le, Fei ; Townsend-Nicholson, Andrea ; Baker, Elizabeth ; Sutherland, Grant R. ; Schofield, Peter R. |
Abstract: | The gene for the stimulatory G protein-coupled human A2a adenosine receptor was isolated and sequence analysis revealed two exons that are interrupted by an intron of approximately 6.4 kb. An intron is located in the same region in the human A1 and A2b adenosine receptor genes. Comparison of the A2a genomic and cDNA sequences reveals two nucleotide differences in the coding region and the presence of an aberrant sequence in the 5'208 base pairs of the A2a cDNA including a polymorphism in the third base of codon Tyr-361 and Gly codon which was always detected at residue 392, indicated that the Arg codon present in the cDNA may be an artifact. Fluorescent in situ hybridization and PCR analysis of human-hamster hybrid cell panels shows that the A2a receptor gene is localized to chromosome 22q11.2. This is in contrast with previous reports (subsequently retracted) which mapped the A2a gene to chromosome 11q11-13. |
Keywords: | Chromosomes, Human, Pair 11 Animals Humans Mice Receptors, Purinergic P1 Receptor, Adenosine A2A DNA, Complementary DNA Primers In Situ Hybridization, Fluorescence Chromosome Mapping Cloning, Molecular Polymerase Chain Reaction Amino Acid Sequence Base Sequence Repetitive Sequences, Nucleic Acid Polymorphism, Genetic Genomic Library Pseudogenes Molecular Sequence Data Cricetinae Male |
DOI: | 10.1006/bbrc.1996.0916 |
Published version: | http://dx.doi.org/10.1006/bbrc.1996.0916 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.