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https://hdl.handle.net/2440/7025
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dc.contributor.author | Hardy, S. | - |
dc.contributor.author | Robinson, B. | - |
dc.contributor.author | Ferrante, A. | - |
dc.contributor.author | Hii, C. | - |
dc.contributor.author | Johnson, D. | - |
dc.contributor.author | Poulos, A. | - |
dc.contributor.author | Murray, A. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Biochemical Journal, 1995; 311(2):689-697 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.issn | 1470-8728 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7025 | - |
dc.description.abstract | Fatty acids with more than 22 carbon atoms (very-long-chain fatty acids; VLCFAs) are normal cellular components that have been implicated in the pathophysiology of a number of peroxisomal disorders. To date, however, essentially nothing is known regarding their biological activities. Ca2+ mobilization is an important intracellular signalling system for a variety of agonists and cell types. Given that several polyunsaturated long-chain fatty acids mobilize intracellular Ca2+ and that we have postulated that the VLCFAs may be involved in signal transduction, we examined whether the tetraenoic VLCFA induced Ca2+ mobilization in human neutrophils. We report that fatty acid-induced intracellular Ca2+ mobilization declined for fatty acid species of more than 20 carbon atoms, but increased again as the carbon chain length approached 30. This Ca2+ mobilization occurred independently of inositol 1,4,5-triphosphate production and protein kinase C translocation and involved both the release of Ca2+ from the intracellular stores and changes to the influx or efflux of the ion. We further observed that triacontatetraenoic acid [30:4(n-6)] mobilized Ca2+ from a thapsigargin-insensitive intracellular pool distinct from the thapsigargin-sensitive pools affected by arachidonic acid [20:4(n - 6)] or N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). 20:4 (n - 6) induced strong superoxide production (chemiluminescence) which was inhibited by thapsigargin pretreatment. In contrast, fatty acid-induced superoxide production progressively declined as the carbon chain length increased beyond 20-22 carbon atoms. Further studies suggested that the thapsigargin-insensitive Ca2+ mobilization elicited by 30:4 (n - 6) was not related to oxyradical formation, while the thapsigargin-sensitive Ca2+ mobilization induced by 20:4 (n - 6) may be involved in the initiation but not necessarily the maintenance of superoxide production. In conclusion, this is the first report to demonstrate a biological activity for the VLCFA and indicates that 30:4 (n - 6) influences second messenger systems in intact cells that differ from those affected by long-chain fatty acids such as 20:4 (n - 6). | - |
dc.description.statementofresponsibility | S J Hardy, B S Robinson, A Ferrante, C S Hii, D W Johnson, A Poulos and A W Murray | - |
dc.language.iso | en | - |
dc.publisher | PORTLAND PRESS | - |
dc.source.uri | http://www.biochemj.org.proxy.library.adelaide.edu.au/bj/311/bj3110689.htm | - |
dc.subject | Neutrophils | - |
dc.subject | Humans | - |
dc.subject | Superoxides | - |
dc.subject | Calcium | - |
dc.subject | Inositol 1,4,5-Trisphosphate | - |
dc.subject | Terpenes | - |
dc.subject | Thapsigargin | - |
dc.subject | Fura-2 | - |
dc.subject | Protein Kinase C | - |
dc.subject | Fatty Acids | - |
dc.subject | Ovalbumin | - |
dc.subject | Serum Albumin, Bovine | - |
dc.subject | Enzyme Inhibitors | - |
dc.subject | Luminescent Measurements | - |
dc.subject | Second Messenger Systems | - |
dc.subject | Structure-Activity Relationship | - |
dc.subject | Calcium-Transporting ATPases | - |
dc.title | Polyenoic very-long-chain fatty acids mobilise calcium from a thapsigargin-insensitive pool in human neutrophils. The relationship between Ca++ mobilization and superoxide production induced by long- and very-long fatty acids | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1042/bj3110689 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ferrante, A. [0000-0002-2581-6407] | - |
dc.identifier.orcid | Hii, C. [0000-0002-7107-8935] | - |
Appears in Collections: | Aurora harvest Paediatrics publications |
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