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https://hdl.handle.net/2440/70528
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Type: | Journal article |
Title: | Rice DUR3 mediates high-affinity urea transport and plays an effective role in improvement of urea acquisition and utilization when expressed in Arabidopsis |
Author: | Wang, W. Kohler, B. Cao, F. Liu, G. Gong, Y. Sheng, S. Song, Q. Cheng, X. Garnett, T. Okamoto, M. Qin, R. Mueller-Roeber, B. Tester, M. Liu, L. |
Citation: | New Phytologist, 2012; 193(2):432-444 |
Publisher: | Blackwell Publishing Ltd |
Issue Date: | 2012 |
ISSN: | 0028-646X 1469-8137 |
Organisation: | Australian Centre for Plant Functional Genomics (ACPFG) |
Statement of Responsibility: | Wei-Hong Wang, Barbara Köhler, Feng-Qiu Cao, Guo-Wei Liu, Yuan-Yong Gong, Song Sheng, Qi-Chao Song, Xiao-Yuan Cheng, Trevor Garnett, Mamoru Okamoto, Rui Qin, Bernd Mueller-Roeber, Mark Tester, and Lai-Hua Liu |
Abstract: | • Despite the great agricultural and ecological importance of efficient use of urea-containing nitrogen fertilizers by crops, molecular and physiological identities of urea transport in higher plants have been investigated only in Arabidopsis. • We performed short-time urea-influx assays which have identified a low-affinity and high-affinity (K(m) of 7.55 μM) transport system for urea-uptake by rice roots (Oryza sativa). • A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops. OsDUR3 encodes an integral membrane-protein with 721 amino acid residues and 15 predicted transmembrane domains. Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a K(m) of c. 10 μM in Xenopus oocytes. • Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation. Importantly, overexpression of OsDUR3 complemented the Arabidopsis atdur3-1 mutant, improving growth on low urea and increasing root urea-uptake markedly. Together with its plasma membrane localization detected by green fluorescent protein (GFP)-tagging and with findings that disruption of OsDUR3 by T-DNA reduces rice growth on urea and urea uptake, we suggest that OsDUR3 is an active urea transporter that plays a significant role in effective urea acquisition and utilisation in rice. |
Keywords: | high-affinity transporter leaf senescence nitrogen remobilization OsDUR3 overexpression rice plant urea transport and utilization. |
Rights: | © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust |
DOI: | 10.1111/j.1469-8137.2011.03929.x |
Published version: | http://dx.doi.org/10.1111/j.1469-8137.2011.03929.x |
Appears in Collections: | Aurora harvest Australian Centre for Plant Functional Genomics publications |
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