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https://hdl.handle.net/2440/7133
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Type: | Journal article |
Title: | Rapid and simultaneous quantification of rhamnose, mannitol, and lactulose in urine by HPLC for estimating intestinal permeability in pediatric practice |
Author: | Miki, K. Butler, R. Moore, D. Davidson, G. |
Citation: | Clinical Chemistry (Washington, DC): international journal of molecular diagnostics and laboratory medicine, 1996; 42(1):71-75 |
Publisher: | Oxford University Press (OUP) |
Issue Date: | 1996 |
ISSN: | 0009-9147 1530-8561 |
Statement of Responsibility: | Miki, K; Butler, R; Moore, D; Davidson, G |
Abstract: | Determination of intestinal permeability by measuring nonmetabolized sugars has been used to assess the integrity of intestinal mucosa. We have developed and validated a modified HPLC method for determining the concentration of L-rhamnose, mannitol, and lactulose in urine, using an amine-modified silica column and refractive index detection. Probe sugars are simultaneously resolved within 18 min. The calibration curve for each sugar is linear to 20 mmol/L. The minimum detectable concentration of lactulose is 0.05 mmol/L. Recovery of probe sugars is between 99.3% and 105.1%. Overall precision (CV) of estimation of probe sugars ranges from 4.2% to 6.5%. In 14 urine samples from healthy children who ingested the test solution containing 1 g of L-rhamnose, 1 g of mannitol, and 5 g of lactulose, the 5-h urinary excretion ratios of lactulose/rhamnose and lactulose/mannitol were 0.047 +/- 0.018 and 0.021 +/- 0.010 (mean +/- SD), respectively. This method presents a rapid, convenient, and practical technique for determining intestinal permeability in clinical pediatric practice. |
Keywords: | Intestines Humans Mannitol Rhamnose Lactulose Chromatography, High Pressure Liquid Permeability Reference Values Adolescent Child |
Rights: | © 1996 The American Association for Clinical Chemistry |
DOI: | 10.1093/clinchem/42.1.71 |
Published version: | http://www.clinchem.org/content/42/1/71.abstract |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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