Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/7133
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dc.contributor.author | Miki, K. | - |
dc.contributor.author | Butler, R. | - |
dc.contributor.author | Moore, D. | - |
dc.contributor.author | Davidson, G. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Clinical Chemistry (Washington, DC): international journal of molecular diagnostics and laboratory medicine, 1996; 42(1):71-75 | - |
dc.identifier.issn | 0009-9147 | - |
dc.identifier.issn | 1530-8561 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7133 | - |
dc.description.abstract | Determination of intestinal permeability by measuring nonmetabolized sugars has been used to assess the integrity of intestinal mucosa. We have developed and validated a modified HPLC method for determining the concentration of L-rhamnose, mannitol, and lactulose in urine, using an amine-modified silica column and refractive index detection. Probe sugars are simultaneously resolved within 18 min. The calibration curve for each sugar is linear to 20 mmol/L. The minimum detectable concentration of lactulose is 0.05 mmol/L. Recovery of probe sugars is between 99.3% and 105.1%. Overall precision (CV) of estimation of probe sugars ranges from 4.2% to 6.5%. In 14 urine samples from healthy children who ingested the test solution containing 1 g of L-rhamnose, 1 g of mannitol, and 5 g of lactulose, the 5-h urinary excretion ratios of lactulose/rhamnose and lactulose/mannitol were 0.047 +/- 0.018 and 0.021 +/- 0.010 (mean +/- SD), respectively. This method presents a rapid, convenient, and practical technique for determining intestinal permeability in clinical pediatric practice. | - |
dc.description.statementofresponsibility | Miki, K; Butler, R; Moore, D; Davidson, G | - |
dc.language.iso | en | - |
dc.publisher | Oxford University Press (OUP) | - |
dc.rights | © 1996 The American Association for Clinical Chemistry | - |
dc.source.uri | http://www.clinchem.org/content/42/1/71.abstract | - |
dc.subject | Intestines | - |
dc.subject | Humans | - |
dc.subject | Mannitol | - |
dc.subject | Rhamnose | - |
dc.subject | Lactulose | - |
dc.subject | Chromatography, High Pressure Liquid | - |
dc.subject | Permeability | - |
dc.subject | Reference Values | - |
dc.subject | Adolescent | - |
dc.subject | Child | - |
dc.title | Rapid and simultaneous quantification of rhamnose, mannitol, and lactulose in urine by HPLC for estimating intestinal permeability in pediatric practice | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/clinchem/42.1.71 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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