Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/71520
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEbneter, A.en
dc.contributor.authorCasson, R.en
dc.contributor.authorWood, J.en
dc.contributor.authorChidlow, G.en
dc.date.issued2012en
dc.identifier.citationClinical and Experimental Ophthalmology, 2012; 40(6):626-633en
dc.identifier.issn1442-6404en
dc.identifier.issn1442-9071en
dc.identifier.urihttp://hdl.handle.net/2440/71520-
dc.description.abstractBackground:  Full axon counting of optic nerve cross-sections represents the most accurate method to quantify axonal damage, but such analysis is very labour intensive. Recently, a new method has been developed, termed targeted sampling, which combines the salient features of a grading scheme with axon counting. Preliminary findings revealed the method compared favourably with random sampling. The aim of the current study was to advance our understanding of the effect of sampling patterns on axon counts by comparing estimated axon counts from targeted sampling with those obtained from fixed-pattern sampling in a large collection of optic nerves with different severities of axonal injury. Methods:  Chronic ocular hypertension was induced in adult Sprague-Dawley rats for 1–7 weeks by translimbal laser photocoagulation of the trabecular meshwork. Axonal damage on resin-embedded cross-sections was estimated using three different methods: (i) semi-quantitative damage grading; (ii) semi-quantitative, automated axon counting using targeted sampling; and (iii) semi-quantitative, automated axon counting using fixed-pattern sampling. Results:  Estimated axon counts, as generated by targeted sampling and fixed-pattern sampling, correlated equally well with the semi-quantitative grading scheme. Estimated counts obtained with targeted sampling were not statistically different from those yielded by fixed-pattern sampling. Bland–Altman analysis showed a good agreement between the two methods. Conclusions:  The results of our study validate the use of both fixed-pattern sampling and targeted sampling for estimation of axonal damage but do not indicate that the latter method is superior for detection of axon loss in animals with minor damage.en
dc.description.statementofresponsibilityAndreas Ebneter, Robert J Casson, John PM Wood and Glyn Chidlowen
dc.language.isoenen
dc.publisherBlackwell Publishing Asiaen
dc.rights© 2011 The Authors. Clinical and Experimental Ophthalmology © 2011 Royal Australian and New Zealand College of Ophthalmologistsen
dc.subjectaxon; experimental glaucoma; nerve degeneration; optic nerve.en
dc.titleEstimation of axon counts in a rat model of glaucoma: comparison of fixed-pattern sampling with targeted samplingen
dc.typeJournal articleen
dc.identifier.rmid0020117840en
dc.identifier.doi10.1111/j.1442-9071.2011.02741.xen
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/565202en
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/626964en
dc.identifier.pubid25182-
pubs.library.collectionOpthalmology & Visual Sciences publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidCasson, R. [0000-0003-2822-4076]en
Appears in Collections:Opthalmology & Visual Sciences publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.