Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/72540
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKumeria, T.en
dc.contributor.authorKurkuri, M.en
dc.contributor.authorDiener, K.en
dc.contributor.authorParkinson, L.en
dc.contributor.authorLosic, D.en
dc.date.issued2012en
dc.identifier.citationBiosensors & Bioelectronics, 2012; 35(1):167-173en
dc.identifier.issn0956-5663en
dc.identifier.issn1873-4235en
dc.identifier.urihttp://hdl.handle.net/2440/72540-
dc.description.abstractIn this report, a label-free reflectometric interference spectroscopy (RIfS) based microchip biosensor for the detection of circulating tumour cells (CTCs) is demonstrated. Highly ordered nanoporous anodic aluminium oxide (AAO) fabricated by electrochemical anodization of aluminium foil was used as the RIfS sensing platform. Biotinylated anti-EpCAM antibody that specifically binds to human cancer cells of epithelial origin such as pancreatic cancer cells (PANC-1) was covalently attached to the AAO surface through multiple surface functionalization steps. Whole blood or phosphate buffer saline spiked with low numbers of pancreatic cancer cells were successfully detected by specially designed microfluidic device incorporating an AAO RIfS sensor, without labour intensive fluorescence labelling and/or pre-enhancement process. Our results show that the developed device is capable of selectively detecting of cancer cells, within a concentrations range of 1000-100,000 cells/mL, with a detection limit of <1000 cells/mL, a response time of <5 min and sample volume of 50 μL of. The presented RIfS method shows considerable promise for translation to a rapid and cost-effective point-of-care diagnostic device for the detection of CTCs in patients with metastatic cancer.en
dc.description.statementofresponsibilityTushar Kumeria, Mahaveer D. Kurkuri, Kerrilyn R. Diener, Luke Parkinson and Dusan Losicen
dc.language.isoenen
dc.publisherElsevier Advanced Technologyen
dc.rightsCrown Copyright © 2012 Published by Elsevier B.V. All rights reserved.en
dc.subjectCell Line, Tumor; Humans; Aluminum Oxide; Cell Adhesion Molecules; Antibodies, Neoplasm; Antigens, Neoplasm; Microscopy, Electron, Scanning; Microfluidic Analytical Techniques; Spectrum Analysis; Equipment Design; Biosensing Techniques; Surface Properties; Porosity; Metal Nanoparticles; Neoplastic Cells, Circulating; Antibodies, Immobilized; Lab-On-A-Chip Devices; Limit of Detection; Epithelial Cell Adhesion Moleculeen
dc.titleLabel-free reflectometric interference microchip biosensor based on nanoporous alumina for detection of circulating tumour cellsen
dc.typeJournal articleen
dc.identifier.rmid0020118967en
dc.identifier.doi10.1016/j.bios.2012.02.038en
dc.identifier.pubid24584-
pubs.library.collectionChemical Engineering publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidDiener, K. [0000-0001-8417-5542]en
dc.identifier.orcidLosic, D. [0000-0002-1930-072X]en
Appears in Collections:Chemical Engineering publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.