Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7270
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBaker, R.en
dc.contributor.authorWang, X.en
dc.contributor.authorWoollatt, E.en
dc.contributor.authorWhite, J.en
dc.contributor.authorSutherland, G.en
dc.date.issued1999en
dc.identifier.citationGenomics, 1999; 59(3):264-274en
dc.identifier.issn0888-7543en
dc.identifier.issn1089-8646en
dc.identifier.urihttp://hdl.handle.net/2440/7270-
dc.description.abstractWe have identified a novel gene, USP15, encoding a human ubiquitin-specific protease (USP). The USP15 protein consists of 952 amino acids with a predicted molecular mass of 109.2 kDa and contains the highly conserved Cys and His boxes present in all members of the UBP family of deubiquitinating enzymes. USP15 shares 60.5% sequence identity and 76% sequence similarity with the human homolog (UNP/Unph/USP4) of the mouse Unp proto-oncogene. Recombinant USP15 demonstrated ubiquitin-specific protease activity against engineered linear fusions of ubiquitin to beta-galactosidase and glutathione S-transferase. USP15 can also cleave the ubiquitin-proline bond, a property previously unique to Unp/UNP. Chromosomal mapping by fluorescence in situ hybridization and radiation hybrid analyses localized the USP15 gene to chromosome band 12q14, a different location than that of UNP (3p21.3). Analysis of expressed sequence tag databases reveals evidence of alternate polyadenylation sites in the USP15 gene and also indicates that the gene may possess an exon/intron structure similar to that of the Unp gene, suggesting they have descended from a common ancestor. A systematic nomenclature for the human USPs is proposed.en
dc.description.statementofresponsibilityRohan T. Baker, Xiao-Wen Wang, Erica Woollatt, Julia A. White, Grant R. Sutherlanden
dc.language.isoenen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen
dc.subjectChromosomes, Human, Pair 12; Animals; Humans; Mice; Ubiquitin Thiolesterase; Endopeptidases; Proline; Avian Proteins; DNA-Binding Proteins; Trans-Activators; Oncogene Proteins; Proto-Oncogene Proteins; Transcription Factors; Ubiquitins; DNA, Complementary; Chromosome Mapping; Cloning, Molecular; Amino Acid Sequence; Base Sequence; Sequence Homology, Amino Acid; Molecular Sequence Data; MafB Transcription Factor; Terminology as Topic; Ubiquitin-Specific Proteasesen
dc.titleIdentification, functional characterization, and chromosomal localization of USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, and a systematic nomenclature for human ubiquitin-specific proteasesen
dc.typeJournal articleen
dc.identifier.rmid0030005640en
dc.identifier.doi10.1006/geno.1999.5879en
dc.identifier.pubid69646-
pubs.library.collectionPaediatrics publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.