Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7288
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Type: Journal article
Title: Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25
Author: Grossman, A.
Mittrucker, H.
Nicholl, J.
Suzuki, A.
Chung, S.
Antonio, L.
Suggs, S.
Sutherland, G.
Siderovski, D.
Mak, T.
Citation: Genomics, 1996; 37(2):229-233
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Issue Date: 1996
ISSN: 0888-7543
1089-8646
Statement of
Responsibility: 
Grossman, Alex ; Mittrücker, Hans-Willi ; Nicholl, Jillian ; Suzuki, Akira ; Chung, Stephen ; Antonio, Laarni ; Suggs, Sid ; Sutherland, Grant R. ; Siderovski, David P. ; Mak, Tak W.
Abstract: The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of approximately 5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU-1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25.
Keywords: Hela Cells; Tumor Cells, Cultured; Chromosomes, Human, Pair 6; Animals; Humans; Mice; DNA-Binding Proteins; Transcription Factors; DNA, Complementary; In Situ Hybridization, Fluorescence; Chromosome Mapping; Cloning, Molecular; Binding Sites; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Interferon Regulatory Factors; Promoter Regions, Genetic
RMID: 0030005633
DOI: 10.1006/geno.1996.0547
Appears in Collections:Paediatrics publications

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