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https://hdl.handle.net/2440/7288
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dc.contributor.author | Grossman, A. | - |
dc.contributor.author | Mittrucker, H.W. | - |
dc.contributor.author | Nicholl, J. | - |
dc.contributor.author | Suzuki, A. | - |
dc.contributor.author | Chung, S. | - |
dc.contributor.author | Antonio, L. | - |
dc.contributor.author | Suggs, S. | - |
dc.contributor.author | Sutherland, G. | - |
dc.contributor.author | Siderovski, D. | - |
dc.contributor.author | Mak, T. | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | Genomics, 1996; 37(2):229-233 | - |
dc.identifier.issn | 0888-7543 | - |
dc.identifier.issn | 1089-8646 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7288 | - |
dc.description.abstract | The interferon regulatory factor (IRF) genes encode a family of transcription factors involved in the transcriptional regulation of interferon and the interferon stimulated genes through recognition of the interferon stimulated response element. We previously reported the cloning of a murine lymphocyte-specific IRF (mLSIRF), which was rapidly induced following B- or T-cell receptor crosslinking. To study the role of LSIRF in human lymphocyte development, we have cloned the complete 5.3-kb cDNA for the human homolog (hLSIRF). hLSIRF is a protein of 450 amino acids with a predicted molecular weight of 51.6 kDa and possesses 92% identity at the amino acid level to mLSIRF, including near identity in the DNA-binding domain. In Northern blot analysis, a single transcript of approximately 5 kb was highly expressed in spleen and peripheral blood lymphocyte. hLSIRF mRNA was rapidly induced in peripheral T cells after crosslinking the T-cell receptor. Analysis of tumor cell lines showed that hLSIRF mRNA was basally expressed in most B- but not T-cell lines. Surprisingly hLSIRF mRNA was also found in the melanoma line G361 and is expressed in normal melanocytes as well. Sequence from a genomic clone for hLSIRF was compared to that from mouse and revealed an identical exon-intron structure and a conserved PU-1-binding motif in the promoter. By FISH analysis, hLSIRF was mapped to 6p23-p25. | - |
dc.description.statementofresponsibility | Grossman, Alex ; Mittrücker, Hans-Willi ; Nicholl, Jillian ; Suzuki, Akira ; Chung, Stephen ; Antonio, Laarni ; Suggs, Sid ; Sutherland, Grant R. ; Siderovski, David P. ; Mak, Tak W. | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | - |
dc.source.uri | http://dx.doi.org/10.1006/geno.1996.0547 | - |
dc.subject | Hela Cells | - |
dc.subject | Tumor Cells, Cultured | - |
dc.subject | Chromosomes, Human, Pair 6 | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | DNA-Binding Proteins | - |
dc.subject | Transcription Factors | - |
dc.subject | DNA, Complementary | - |
dc.subject | In Situ Hybridization, Fluorescence | - |
dc.subject | Chromosome Mapping | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Binding Sites | - |
dc.subject | Amino Acid Sequence | - |
dc.subject | Base Sequence | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Interferon Regulatory Factors | - |
dc.subject | Promoter Regions, Genetic | - |
dc.title | Cloning of human lymphocyte-specific interferon regulatory factor (hLSIRF/hIRF4) and mapping of the gene to 6p23-p25 | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1006/geno.1996.0547 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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