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Type: Journal article
Title: Generation of murine monoclonal antibodies to Haemophilus influenzae type b capsular polysaccharide by in vivo immunization
Author: Kodituwakku, A.
Zola, H.
Roberton, D.
Citation: Hybridoma, 2004; 23(3):160-167
Publisher: Mary Ann Liebert, Inc. Publishers
Issue Date: 2004
ISSN: 1554-0014
Abstract: Haemophilus influenzae type b (Hib) is a pathogenic gram-negative bacterium associated with human disease, especially in young children. Protective immune response to Hib results from antibodies developed against the polyribosylribitol phosphate (PRP) capsular polysaccharide of the bacterium. Several investigators in the study of immune response to Hib have produced human monoclonal antibodies to PRP. Only two previous groups have reported the generation of murine anti-PRP monoclonal antibodies using either immunizing mice with inactivated Hib bacteria or a combination of in vivo and in vitro immunization of mice with PRP conjugate antigen (PRP-D). In this present study, we generated murine anti-PRP monoclonal antibody secreting hybridomas for the first time by simple in vivo immunization with PRP conjugate antigen (PRP-T). The anti-PRP antibodies from one hybridoma clone (B10) are further characterized and potential applications are discussed.
Keywords: Hybridomas; Animals; Mice; Haemophilus influenzae type b; Polysaccharides, Bacterial; Bacterial Capsules; Haemophilus Vaccines; Antibodies, Monoclonal; Immunoglobulin Isotypes; Enzyme-Linked Immunosorbent Assay; Agglutination Tests; Immunization; Luminescent Measurements; Microbial Sensitivity Tests
RMID: 0020041823
DOI: 10.1089/1536859041224325
Appears in Collections:Paediatrics publications

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