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https://hdl.handle.net/2440/73757
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Type: | Journal article |
Title: | Purification and characterization of alkaline pectin lyase from a newly isolated Bacillus clausii and its application in elicitation of plant disease resistance |
Author: | Li, Z. Bai, Z. Zhang, B. Li, B. Jin, B. Zhang, M. Lin, F. Zhang, H. |
Citation: | Applied Biochemistry and Biotechnology, 2012; 167(8):2241-2256 |
Publisher: | Humana Press Inc |
Issue Date: | 2012 |
ISSN: | 0273-2289 1559-0291 |
Statement of Responsibility: | Zuming Li, Zhihui Bai, Baoguo Zhang, Baojv Li, Bo Jin, Michael Zhang, Francis Lin and Hongxun Zhang |
Abstract: | Alkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K m of 0.87 mg/ml at pH 10.0 and 60 °C. The enzyme is stable in the pH range of 8.0–10.0 and temperature ≤40 °C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture. |
Keywords: | Bacillus clausii Alkaline pectin lyase sugar beet pulp mass spectrometric sequencing plant disease resistance |
Rights: | © Springer Science+Business Media, LLC 2012 |
DOI: | 10.1007/s12010-012-9758-9 |
Published version: | http://dx.doi.org/10.1007/s12010-012-9758-9 |
Appears in Collections: | Aurora harvest Chemical Engineering publications Environment Institute publications |
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