Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/74777
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Type: Journal article
Title: Isolation and molecular characterization of vancomycin-resistant enterococcus faecium from swine in Michigan, USA
Author: Gordoncillo, M.
Donabedian, S.
Bartlett, P.
Perri, M.
Zervos, M.
Kirkwood, R.
Febvay, C.
Citation: Zoonoses and Public Health, 2013; 60(5):319-326
Publisher: Wiley-Blackwell Verlag GmbH
Issue Date: 2013
ISSN: 1863-2378
Statement of
Responsibility: 
M. J. N. Gordoncillo, S. Donabedian, P. C. Bartlett, M. Perri, M. Zervos, R. Kirkwood and C. Febvay
Abstract: In 2008, we identified vancomycin-resistant enterococci (VRE) in Michigan swine, which was the first report of VRE in livestock from North America. Continued sampling in 2009 and 2010 was conducted to determine whether VRE persisted in Michigan. In 2009, swine faecal and feed samples (n = 56), county fair pig barn manure samples (n = 9) and pooled Michigan State Fair pig barn manure samples (n = 18) were screened for VRE. In 2010, swine faecal samples were collected from 26 county fairs (n = 73) and nine commercial swine farms in six states (n = 28). Recovered VRE isolates were molecularly evaluated by polymerase chain reaction, restriction fragment length polymorphism, pulsed-field gel electrophoresis (PFGE), S1 nuclease digestion and multilocus sequence typing (MLST). Six VRE isolates were identified in 2009 from the State Fair, and another six (8.2%) were recovered from the five county fairs in 2010. All 12 isolates were highly related to the first-reported VRE from Michigan swine: all were confirmed to be vancomycin-resistant Enterococcus faecium (VREf) carrying vanA gene on Tn1546 (Type D), were negative for IS1251, hyl and esp gene, carried a 150–160 kb megaplasmid, and have closely similar PFGE patterns with >80% similarity. Classified as ST5, ST6 or ST185 by MLST, all belong to the clonal complex 5, a strain recognized to be circulating among European pigs. This study reveals that VREf are widespread in Michigan swine and persist in the historical absence of the use of agricultural glycopeptides.
Keywords: Vancomycin-resistant enterococci; vancomycin-resistant Enterococcus faecium; swine; Michigan; vanA gene; clonal complex
Rights: © 2012 Blackwell Verlag GmbH
RMID: 0020122796
DOI: 10.1111/zph.12008
Appears in Collections:Animal and Veterinary Sciences publications

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