Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/74830
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Type: | Journal article |
Title: | ZEB1 drives prometastatic actin cytoskeletal remodeling by downregulating miR-34a expression |
Author: | Ahn, Y. Gibbons, D. Chakravarti, D. Creighton, C. Rizvi, Z. Adams, H. Pertsemlidis, A. Gregory, P. Wright, J. Goodall, G. Flores, E. Kurie, J. |
Citation: | Journal of Clinical Investigation, 2012; 122(9):3170-3183 |
Publisher: | Amer Soc Clinical Investigation Inc |
Issue Date: | 2012 |
ISSN: | 0021-9738 1558-8238 |
Statement of Responsibility: | Young-Ho Ahn, Don L. Gibbons, Deepavali Chakravarti, Chad J. Creighton, Zain H. Rizvi, Henry P. Adams, Alexander Pertsemlidis, Philip A. Gregory, Josephine A. Wright, Gregory J. Goodall, Elsa R. Flores and Jonathan M. Kurie |
Abstract: | Metastatic cancer is extremely difficult to treat, and the presence of metastases greatly reduces a cancer patient’s likelihood of long-term survival. The ZEB1 transcriptional repressor promotes metastasis through downregulation of microRNAs (miRs) that are strong inducers of epithelial differentiation and inhibitors of stem cell factors. Given that each miR can target multiple genes with diverse functions, we posited that the prometastatic network controlled by ZEB1 extends beyond these processes. We tested this hypothesis using a mouse model of human lung adenocarcinoma metastasis driven by ZEB1, human lung carcinoma cells, and human breast carcinoma cells. Transcriptional profiling studies revealed that ZEB1 controls the expression of numerous oncogenic and tumor-suppressive miRs, including miR-34a. Ectopic expression of miR-34a decreased tumor cell invasion and metastasis, inhibited the formation of promigratory cytoskeletal structures, suppressed activation of the RHO GTPase family, and regulated a gene expression signature enriched in cytoskeletal functions and predictive of outcome in human lung adenocarcinomas. We identified several miR-34a target genes, including Arhgap1, which encodes a RHO GTPase activating protein that was required for tumor cell invasion. These findings demonstrate that ZEB1 drives prometastatic actin cytoskeletal remodeling by downregulating miR-34a expression and provide a compelling rationale to develop miR-34a as a therapeutic agent in lung cancer patients. |
Keywords: | Cells, Cultured Cell Line, Tumor Animals Humans Mice Adenocarcinoma Lung Neoplasms Doxycycline rho GTP-Binding Proteins Luciferases Transforming Growth Factor beta Homeodomain Proteins Tumor Suppressor Proteins Transcription Factors MicroRNAs Oligonucleotide Array Sequence Analysis Cell Separation Statistics, Nonparametric Neoplasm Transplantation Signal Transduction Cell Proliferation Cell Movement Down-Regulation Gene Expression Regulation, Neoplastic Genes, Reporter Promoter Regions, Genetic Mice, 129 Strain Epithelial-Mesenchymal Transition Actin Cytoskeleton Transcriptome Zinc Finger E-box-Binding Homeobox 1 |
Rights: | Copyright © 2012, American Society for Clinical Investigation |
DOI: | 10.1172/JCI63608 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1008327 R01 CA157450 P30 CA125123 |
Published version: | http://dx.doi.org/10.1172/jci63608 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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File | Description | Size | Format | |
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hdl_74830.pdf | Published version | 5.07 MB | Adobe PDF | View/Open |
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