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Type: Journal article
Title: Determining the polysaccharide composition of plant cell walls
Author: Pettolino, F.
Walsh, C.
Fincher, G.
Bacic, A.
Citation: Nature Protocols (Print), 2012; 7(9):1590-1607
Publisher: Nature Publishing Group
Issue Date: 2012
ISSN: 1754-2189
Statement of
Filomena A Pettolino, Cherie Walsh, Geoffrey B Fincher & Antony Bacic
Abstract: The plant cell wall is a chemically complex structure composed mostly of polysaccharides. Detailed analyses of these cell wall polysaccharides are essential for our understanding of plant development and for our use of plant biomass (largely wall material) in the food, agriculture, fabric, timber, biofuel and biocomposite industries. We present analytical techniques not only to define the fine chemical structures of individual cell wall polysaccharides but also to estimate the overall polysaccharide composition of cell wall preparations. The procedure covers the preparation of cell walls, together with gas chromatography–mass spectrometry (GC-MS)-based methods, for both the analysis of monosaccharides as their volatile alditol acetate derivatives and for methylation analysis to determine linkage positions between monosaccharide residues as their volatile partially methylated alditol acetate derivatives. Analysis time will vary depending on both the method used and the tissue type, and ranges from 2 d for a simple neutral sugar composition to 2 weeks for a carboxyl reduction/methylation linkage analysis.
Keywords: Cell Wall; Plants; Polysaccharides; Methylation; Gas Chromatography-Mass Spectrometry; Analytic Sample Preparation Methods
Rights: © 2012 Nature America, Inc. All rights reserved.
RMID: 0020122051
DOI: 10.1038/nprot.2012.081
Appears in Collections:Agriculture, Food and Wine publications

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