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https://hdl.handle.net/2440/75228
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Type: | Journal article |
Title: | Neonatal protein kinase C zeta expression determines the neonatal T-Cell cytokine phenotype and predicts the development and severity of infant allergic disease |
Author: | D'Vaz, N. Ma, Y. Dunstan, J. Lee-Pullen, T. Hii, C. Meldrum, S. Zhang, G. Metcalfe, J. Ferrante, A. Prescott, S. |
Citation: | Allergy, 2012; 67(12):1511-1518 |
Publisher: | Munksgaard Int Publ Ltd |
Issue Date: | 2012 |
ISSN: | 0105-4538 1398-9995 |
Statement of Responsibility: | N. D’Vaz, Y. Ma, J. A. Dunstan, T. F. Lee-Pullen, C. Hii , S. Meldrum, G. Zhang, J. Metcalfe, A. Ferrante, & S. L. Prescott |
Abstract: | Background: Previous studies have demonstrated that reduced T-cell protein kinase C zeta (PKCζ) expression is associated with allergy development in infants born to atopic mothers. This study examined whether this relationship extends to a general population and addressed the basis for the association. Methods: A flow cytometry assay was developed for the measurement of T-cell PKCζ levels in PBMC, cord blood mononuclear cell and whole blood. Cord blood T-cell PKCζ levels were measured in 135 neonates, and allergic disease was evaluated by skin prick test and clinical examination at 12 months of age. Results: Allergic children (particularly those with eczema) had significantly lower neonatal T-cell PKCζ expression than nonallergic children (P < 0.001). PKCζ levels predicted allergic disease with optimal specificity of 86% and sensitivity of 54%. The sensitivity was increased in the children of allergic mothers, who had significantly lower PKC levels than the children of nonallergic mothers. Cord blood PKCζ levels did not affect T-cell maturation in culture as assessed by CD45RA/RO expression, but low PKCζ expression was associated with reduced capacity for IFNc production by matured T cells. Low cord blood PKC expression was further associated with increased IL-13 responses at 6 months. Conclusions: The findings suggest a potential role for the use of PKCζ levels in cord blood T cells as a presymptomatic test to predict allergy risk in children, particularly offspring of allergic mothers, and that the basis of this relationship is related to cytokine patterns in mature T cells. |
Keywords: | allergy prevention atopic dermatitis cord blood cytokines neonatal screening tool neonate predictive markers Protein kinase C zeta sensitization |
Rights: | © 2012 John Wiley & Sons A/S |
DOI: | 10.1111/all.12027 |
Grant ID: | NHMRC |
Published version: | http://dx.doi.org/10.1111/all.12027 |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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