Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/76120
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Characterization of the Nrt2.6 gene in arabidopsis thaliana: a link with plant response to biotic and abiotic stress |
Author: | Dechorgnat, J. Patrit, O. Krapp, A. Fagard, M. Daniel-Vedele, F. |
Citation: | PLoS One, 2012; 7(8):1-11 |
Publisher: | Public Library of Science |
Issue Date: | 2012 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Wu, K. |
Statement of Responsibility: | Julie Dechorgnat, Oriane Patrit, Anne Krapp, Mathilde Fagard and Françoise Daniel-Vedele |
Abstract: | The high affinity nitrate transport system in Arabidopsis thaliana involves one gene and potentially seven genes from the NRT1 and NRT2 family, respectively. Among them, NRT2.1, NRT2.2, NRT2.4 and NRT2.7 proteins have been shown to transport nitrate and are localized on the plasmalemma or the tonoplast membranes. NRT2.1, NRT2.2 and NRT2.4 play a role in nitrate uptake from soil solution by root cells while NRT2.7 is responsible for nitrate loading in the seed vacuole. We have undertaken the functional characterization of a third member of the family, the NRT2.6 gene. NRT2.6 was weakly expressed in most plant organs and its expression was higher in vegetative organs than in reproductive organs. Contrary to other NRT2 members, NRT2.6 expression was not induced by limiting but rather by high nitrogen levels, and no nitrate-related phenotype was found in the nrt2.6-1 mutant. Consistently, the over-expression of the gene failed to complement the nitrate uptake defect of an nrt2.1-nrt2.2 double mutant. The NRT2.6 expression is induced after inoculation of Arabidopsis thaliana by the phytopathogenic bacterium Erwinia amylovora. Interestingly, plants with a decreased NRT2.6 expression showed a lower tolerance to pathogen attack. A correlation was found between NRT2.6 expression and ROS species accumulation in response to infection by E. amylovora and treatment with the redox-active herbicide methyl viologen, suggesting a probable link between NRT2.6 activity and the production of ROS in response to biotic and abiotic stress. |
Keywords: | Erwinia amylovora Arabidopsis Plant Roots Nitrates Paraquat Anion Transport Proteins Arabidopsis Proteins Organ Specificity Plant Diseases Transcription, Genetic Gene Expression Regulation, Plant Biological Transport Oxidative Stress Genotype Mutation Genes, Plant Time Factors Stress, Physiological |
Rights: | Copyright: © Dechorgnat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0042491 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0042491 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_76120.pdf | Published version | 562.92 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.