Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Temporal changes in bone composition, architecture, and strength following estrogen deficiency in osteoporosis
Author: Brennan, O.
Kuliwaba, J.
Lee, T.
Parkinson, I.
Fazzalari, N.
McNamara, L.
O'Brien, F.
Citation: Calcified Tissue International, 2012; 91(6):440-449
Publisher: Springer
Issue Date: 2012
ISSN: 0171-967X
Statement of
Orlaith Brennan, Julia S. Kuliwaba, T. Clive Lee, Ian H. Parkinson, Nicola L. Fazzalari, Laoise M. McNamara, Fergal J. O'Brien
Abstract: Using an ovariectomized (OVX) ovine model, we provide an analysis of the timing of changes in bone following estrogen deficiency. The expression of genes known to regulate osteoclastogenesis, matrix production, and mineralization, as measured by real-time RT-PCR, was significantly increased by 12 months; and increased expression was maintained through to 31 months post-OVX compared to controls. FTIR spectroscopy confirmed that mineralized crystals were less mature than in controls 12 months post-OVX and were even less so by 31 months. The mineral-to-matrix ratio was significantly reduced by 31 months, while the ratio of mature to immature collagen cross-linking was initially increased at 12 months and subsequently reduced at 31 months post-OVX. In contrast, trabecular number, thickness, and separation were unchanged at 12 months. Significant reductions in trabecular number and thickness and a significant increase in trabecular separation were observed 31 months after OVX. Most notably perhaps these combined changes led to a significant reduction in the compressive strength of trabecular bone after 31 months. The results indicate that there is an initial increase in bone turnover, which is accompanied by a change in bone composition. This is followed by a continued increase in bone resorption and relative reduction in bone formation, leading to deterioration in bone microarchitecture. Ultimately, these cumulative changes led to a significant reduction in the compressive strength of bones following 31 months of estrogen deficiency. These findings provide important insight into the time sequence of changes during osteoporosis.
Keywords: Osteoporosis; Microarchitecture; Bone composition; Biomechanical property; Animal model
Rights: © Springer Science+Business Media New York 2012
RMID: 0020123132
DOI: 10.1007/s00223-012-9657-7
Appears in Collections:Pathology publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.