Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/76469
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Exposure to lipid-rich follicular fluid is associated with endoplasmic reticulum stress and impaired oocyte maturation in cumulus-oocyte complexes |
Author: | Yang, X. Wu, L. Chura, L. Liang, X. Lane, M. Norman, R. Robker, R. |
Citation: | Fertility and Sterility, 2012; 97(6):1438-1443 |
Publisher: | Elsevier Science Inc |
Issue Date: | 2012 |
ISSN: | 0015-0282 1556-5653 |
Statement of Responsibility: | Xing Yang, Linda L. Wu, Lindsay R. Chura, Xiaoyan Liang, Michelle Lane, Robert J. Norman, and Rebecca L. Robker |
Abstract: | <h4>Objective</h4>To determine whether the high lipid content of human follicular fluid influences oocyte maturation.<h4>Design</h4>Mouse oocytes as substitutes for human oocytes were exposed to follicular fluids of differing lipid content with outcome monitoring.<h4>Setting</h4>Private infertility clinic and university laboratory.<h4>Patient(s)</h4>Seventy-four women seeking assisted reproduction, and gonadotropin-stimulated mice.<h4>Intervention(s)</h4>Assay of follicular fluids for triglyceride and free fatty acids, and stimulation of mouse cumulus-oocyte complexes (COCs) to maturity in vitro in the presence of lipid-rich or lipid-poor follicular fluid.<h4>Main outcome measure(s)</h4>Oocyte lipid content, expression of endoplasmic reticulum stress marker genes, and oocyte maturation assessed in mouse COCs exposed to lipid-rich follicular fluid were compared with complexes exposed to lipid-poor follicular fluid and complexes matured in vivo.<h4>Result(s)</h4>Follicular fluids were obtained from women of known body mass index undergoing oocyte aspiration at a private infertility clinic, and the follicular fluids were assayed for triglyceride and free fatty acids; those with the highest and lowest levels of these lipids were selected. The mouse COCs exposed to lipid-rich follicular fluid during their maturation had increased oocyte lipid content, induction of endoplasmic reticulum stress markers, and impaired oocyte nuclear maturation.<h4>Conclusion(s)</h4>Increased body mass index is associated with elevated triglycerides and free fatty acids in ovarian follicular fluid. Maturation within this lipid-rich environment is detrimental to oocytes. |
Keywords: | ATF4 ATF6 endoplasmic reticulum stress free fatty acids GRP78 lipotoxicity obesity triglyceride |
Rights: | ©2012 by American Society for Reproductive Medicine. |
DOI: | 10.1016/j.fertnstert.2012.02.034 |
Published version: | http://dx.doi.org/10.1016/j.fertnstert.2012.02.034 |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.