Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/7695
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Type: Journal article
Title: Molecular characterization, pharmacological properties and chromosomal localization of the human GALR2 galanin receptor
Author: Fathi, Z.
Battaglino, P.
Iben, L.
Li, H.
Baker, E.
Zhang, D.
McGovern, R.
Mahle, C.
Sutherland, G.
Iismaa, T.
Dickinson, K.
Antal Zimanyi, I.
Citation: Brain Research, 1998; 58(37653):156-169
Publisher: ELSEVIER SCIENCE BV
Issue Date: 1998
ISSN: 0169-328X
Statement of
Responsibility: 
Fathi, Zahra ; Battaglino, Peter M ; Iben, Lawrence G ; Li, Hui ; Baker, Elizabeth ; Zhang, Donglu ; McGovern, Rachel ; Mahle, Cathy D ; Sutherland, Grant R ; Iismaa, Tiina P ; Dickinson, Kenneth E.J ; Antal Zimanyi, Ildiko
Abstract: The neuropeptide galanin mediates a diverse spectrum of biological activities by interacting with specific G protein-coupled receptors. We have used homology genomic library screening and polymerase chain reaction (PCR) techniques to isolate both genomic and cDNA clones encoding the human homolog of the recently cloned rat GALR2 galanin receptor. By fluorescence in situ hybridization, the gene encoding human GALR2 (GALNR2) has been localized to chromosome 17q25.3. The two coding exons of the human GALNR2 gene, interrupted by an intron positioned at the end of transmembrane domain III, encode a 387 amino acid G protein-coupled receptor with 87% overall amino acid identity with rat GALR2. In HEK-293 cells stably expressing human GALR2, binding of [125I]porcine galanin is saturable and can be displaced by galanin, amino-terminal galanin fragments and chimeric galanin peptides but not by carboxy-terminal galanin fragments. In HEK-293 cells, human GALR2 couples both to Galphaq/11 to stimulate phospholipase C and increase intracellular calcium levels and to Galphai/o to inhibit forskolin-stimulated intracellular cAMP accumulation. A wide tissue distribution is observed by reverse transcriptase (RT)-PCR analysis, with human GALR2 mRNA being detected in many areas of the human central nervous system as well as in peripheral tissues.
Keywords: Cell Line
Chromosomes, Human, Pair 17
Animals
Swine
Humans
Rats
GTP-Binding Proteins
Phosphatidylinositols
Galanin
Receptors, Galanin
Receptors, Neuropeptide
Recombinant Proteins
DNA, Complementary
In Situ Hybridization, Fluorescence
Chromosome Mapping
Cloning, Organism
Transfection
Sequence Alignment
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Kinetics
Molecular Sequence Data
DOI: 10.1016/S0169-328X(98)00116-8
Published version: http://dx.doi.org/10.1016/s0169-328x(98)00116-8
Appears in Collections:Aurora harvest
Paediatrics publications

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