Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/76951
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Type: Journal article
Title: A physical, genetic and functional sequence assembly of the barley genome
Author: Langridge, P.
Shi, B.
Fincher, G.
Citation: Nature, 2012; 491(7426):711-717
Publisher: Nature Publishing Group
Issue Date: 2012
ISSN: 0028-0836
1476-4687
Contributor: Langridge, Peter
Shi, Bu-jun
Fincher, Geoffrey
Statement of
Responsibility: 
The International Barley Genome Sequencing Consortium
Abstract: Barley (Hordeum vulgare L.) is among the world’s earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 ‘high-confidence’ genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
Keywords: International Barley Genome Sequencing Consortium; Hordeum; Crops, Agricultural; Codon, Nonsense; Physical Chromosome Mapping; Sequence Analysis, DNA; Genomics; Evolution, Molecular; Gene Expression Regulation, Plant; Alternative Splicing; Repetitive Sequences, Nucleic Acid; Polymorphism, Single Nucleotide; Genes, Plant; Genome, Plant; Molecular Sequence Annotation; Transcriptome
Description: University of Adelaide contributors: Peter Langridge & Bu-jun Shi for Australian Centre for Plant Functional Genomics, Geoffrey Fincher for ARC Centre of Excellence in Plant Cell Walls
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
RMID: 0020123304
DOI: 10.1038/nature11543
Appears in Collections:Agriculture, Food and Wine publications

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