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|Title:||Gamma-Heregulin: a fusion gene of DOC-4 and neuregulin-1 derived from a chromosome translocation|
|Citation:||Oncogene, 1999; 18(50):7110-7114|
|Xifu Liu, Elizabeth Baker, Helen J Eyre, Grant R Sutherland and Mingdong Zhou|
|Abstract:||gamma-Heregulin was identified as an isoform resulting from alternate splicing of the neuregulin-1 gene, after cloning of its cDNA from the MDA-MB-175 breast cancer cell line. gamma-Heregulin was shown to promote growth of cultured MDA-MB-175 cells resulting from activation of its cognate ErbB tyrosine kinase reporters. We show here that gamma-heregulin is transcribed from a fusion gene resulting from a chromosome translocation in MDA-MB-175 cells. The fusion chromosome is described as dic(8:11)(8qter-->8p12::11q13-->11pter). As a result, the 5' end of the gamma-heregulin gene is derived from the stress-induced gene, DOC-4 (11q13), while the 3' end is from the neuregulin-1 gene (8p12). Thus, expression of gamma-heregulin is under the control of the DOC-4 promoter. By contrast with MDA-MB-175 cells, RT-PCR failed to detect a gamma-heregulin transcript in either E9.5 to E13.5 embryonic mouse tissues, adult mouse tissues or other human tumour cell lines. We conclude, therefore, that gamma-heregulin is not a native isoform of the neuregulin-1 gene, but a novel growth factor that may contribute to tumour cell proliferation.|
|Keywords:||gamma-heregulin; neuregulin-1; DOC-4; gene localization; MDA-MB-175 cell line|
|Appears in Collections:||Paediatrics publications|
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