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https://hdl.handle.net/2440/7762
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DC Field | Value | Language |
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dc.contributor.author | Angus, J. | - |
dc.contributor.author | Thompson, F. | - |
dc.contributor.author | Murphy, K. | - |
dc.contributor.author | Baker, E. | - |
dc.contributor.author | Sutherland, G. | - |
dc.contributor.author | Parsons, P. | - |
dc.contributor.author | Sturm, R. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Oncogene, 1995; 11(4):691-700 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.issn | 1476-5594 | - |
dc.identifier.uri | http://hdl.handle.net/2440/7762 | - |
dc.description.abstract | The Oct transcription factors N-Oct-3 and N-Oct-5 are differentially expressed in normal melanocytes, melanoma tumors and cell lines. We have cloned the human brn-2 gene and have shown that it encodes both the N-Oct-3 and N-Oct-5 octamer binding activities detected in melanoma cells. The brn-2 genomic locus has been mapped to chromosome 6q16 and although chromosomal aberrations are common in this region in melanoma, no deletion or rearrangement of the brn-2 gene in melanoma cell lines was observed. Sequencing of the entire gene showed that there are no intervening sequences within the open reading frame. Antisense RNA-mediated inhibition of brn-2 gene expression in melanoma cells was associated with a change in morphology and loss of melanocytic and neural crest markers, including the melanocyte transcription factor microphthalmia and the TYRP pigmentation genes. In addition, loss of brn-2 in these cells resulted in the complete loss of ability to form tumors in SCID and nu/nu mice. These results suggest roles for brn-2 in the determination of the melanocytic lineage and in the tumorigenic phenotype of melanoma. | - |
dc.language.iso | en | - |
dc.publisher | Macmillan Press | - |
dc.subject | Cell Line | - |
dc.subject | Tumor Cells, Cultured | - |
dc.subject | Chromosomes, Human, Pair 6 | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Mice, SCID | - |
dc.subject | Melanoma | - |
dc.subject | Chromosome Deletion | - |
dc.subject | Homeodomain Proteins | - |
dc.subject | Intercellular Adhesion Molecule-1 | - |
dc.subject | Transcription Factors | - |
dc.subject | RNA, Antisense | - |
dc.subject | DNA, Neoplasm | - |
dc.subject | Blotting, Southern | - |
dc.subject | In Situ Hybridization, Fluorescence | - |
dc.subject | Restriction Mapping | - |
dc.subject | Transfection | - |
dc.subject | Cell Differentiation | - |
dc.subject | Gene Expression | - |
dc.subject | Gene Expression Regulation, Neoplastic | - |
dc.subject | Base Sequence | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | POU Domain Factors | - |
dc.title | The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells | - |
dc.type | Journal article | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 4 Paediatrics publications |
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