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https://hdl.handle.net/2440/77889
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Type: | Book chapter |
Title: | Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice |
Author: | Evrard, A. Bargmann, B. Birnbaum, K. Tester, M. Baumann, U. Johnson, A. |
Citation: | Plant Salt Tolerance: Methods and Protocols, 2012 / Shabala, S., Cuin, T. (ed./s), vol.913, pp.265-276 |
Publisher: | Humana Press |
Publisher Place: | USA |
Issue Date: | 2012 |
Series/Report no.: | Methods in Molecular Biology; 913 |
ISBN: | 9781617799853 |
Editor: | Shabala, S. Cuin, T. |
Statement of Responsibility: | Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson |
Abstract: | Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting. |
Keywords: | Protoplasts Plants, Genetically Modified Arabidopsis Plant Roots Flow Cytometry Cell Separation Germination Salinity Stress, Physiological Plant Cells Oryza Salt Tolerance |
Rights: | © Springer Science+Business Media, LLC 2012 |
DOI: | 10.1007/978-1-61779-986-0_18 |
Published version: | http://dx.doi.org/10.1007/978-1-61779-986-0_18 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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