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https://hdl.handle.net/2440/7801
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Type: | Journal article |
Title: | Activation of the neutrophil bactericidal activity for non-typable Haemophilus influenzae by tumor necrosis factor and lymphotoxin |
Author: | Tan, A.M. Ferrante, A. Goh, D. Roberton, D. Cripps, A. |
Citation: | Pediatric Research, 1995; 37(2):155-159 |
Publisher: | S. Karger |
Issue Date: | 1995 |
ISSN: | 0031-3998 1530-0447 |
Statement of Responsibility: | Anne-Marie Tan, Antonio Ferrante, David H. B. Goh, Don M. Roberton, and Allan W. Cripps |
Abstract: | Previous studies have suggested that, in vivo, activated T lymphocytes and neutrophils are important in immunity to non-typable Haemophilus influenzae. We now extend this work by showing that neutrophils pretreated with products of activated T lymphocytes or activated macrophages show significantly enhanced killing of nontypable H. influenzae. Lymphotoxin, a product of activated T lymphocytes, significantly enhanced the neutrophil-mediated killing of nontypable H. influenzae, and tumor necrosis factor, produced by activated T lymphocytes as well as macrophages stimulated by activated T lymphocytes, also significantly increased the bactericidal activity of neutrophils. These cytokine-induced effects were seen with short pretreatment times of neutrophils and were maximal by 30 min. The killing of H. influenzae by neutrophils required the presence of heat-labile opsonins. In the absence of these opsonins, both tumor necrosis factor and lymphotoxin were unable to promote the killing of the bacteria by neutrophils. Furthermore, the results showed that tumor necrosis factor-primed neutrophils displayed significantly increased expression of CR3 and CR4 that was associated with increased phagocytosis of complement-opsonized nontypable H. influenzae. These cytokines may play an important role in immunity toward nontypable H. influenzae by stimulating neutrophil bactericidal activity. |
Keywords: | Neutrophils Humans Haemophilus influenzae Tumor Necrosis Factor-alpha Integrins Macrophage-1 Antigen Recombinant Proteins Lymphocyte Activation Phagocytosis Macrophage Activation Opsonin Proteins Lymphotoxin-alpha CD11 Antigens |
Rights: | Copyright © 1995 International Pediatric Research Foundation, Inc |
DOI: | 10.1203/00006450-199502000-00005 |
Published version: | http://ovidsp.tx.ovid.com/sp-3.10.0b/ovidweb.cgi?&S=EEBBFPNHHODDBGNHNCNKPFFBFHMAAA00&Link+Set=S.sh.22.23.26.36%7C5%7Csl_10 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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