Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/78990
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Type: Journal article
Title: ER stress causes rapid loss of intestinal epithelial stemness through activation of the unfolded protein response
Author: Heijmans, J.
van Lidth de Jeude, J.
Koo, B.
Rosekrans, S.
Wielenga, M.
van de Wetering, M.
Ferrante, M.
Lee, A.
Onderwater, J.
Paton, J.
Paton, A.
Mommaas, A.
Kodach, L.
Hardwick, J.
Hommes, D.
Clevers, H.
Muncan, V.
van den Brink, G.
Citation: Cell Reports, 2013; 3(4):1128-1139
Publisher: Elsevier Inc
Issue Date: 2013
ISSN: 2211-1247
2211-1247
Statement of
Responsibility: 
Jarom Heijmans, Jooske F. van Lidth de Jeude, Bon-Kyoung Koo, Sanne L. Rosekrans, Mattheus C.B. Wielenga, Marc van de Wetering, Marc Ferrante, Amy S. Lee, Jos J.M. Onderwater, James C. Paton, Adrienne W. Paton, A. Mieke Mommaas, Liudmila L. Kodach, James C. Hardwick, Daniël W. Hommes, Hans Clevers, Vanesa Muncan, and Gijs R. van den Brink
Abstract: Stem cells generate rapidly dividing transit-amplifying cells that have lost the capacity for self-renewal but cycle for a number of times until they exit the cell cycle and undergo terminal differentiation. We know very little of the type of signals that trigger the earliest steps of stem cell differentiation and mediate a stem cell to transit-amplifying cell transition. We show that in normal intestinal epithelium, endoplasmic reticulum (ER) stress and activity of the unfolded protein response (UPR) are induced at the transition from stem cell to transit-amplifying cell. Induction of ER stress causes loss of stemness in a Perk-eIF2α-dependent manner. Inhibition of Perk-eIF2α signaling results in stem cell accumulation in organoid culture of primary intestinal epithelium. Our findings show that the UPR plays an important role in the regulation of intestinal epithelial stem cell differentiation.
Keywords: Intestinal Mucosa; Cells, Cultured; Stem Cells; Animals; Mice; eIF-2 Kinase; Heat-Shock Proteins; Eukaryotic Initiation Factor-2; RNA, Small Interfering; Signal Transduction; Cell Differentiation; RNA Interference; Mutation; Unfolded Protein Response; Endoplasmic Reticulum Stress
Rights: Copyright © 2013 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020128050
DOI: 10.1016/j.celrep.2013.02.031
Appears in Collections:Molecular and Biomedical Science publications

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