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Type: Journal article
Title: Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing
Author: Li, X.
Zhu, X.
Zhang, C.
Cathro, P.
Seneviratne, C.
Shen, S.
Citation: Chinese Medical Journal, 2013; 126(4):634-639
Publisher: Chinese Medical Association
Issue Date: 2013
ISSN: 0366-6999
Statement of
Li Xin, Zhu Xiao-Fei, Zhang Cheng-Fei, Peter Cathro, Cj Seneviratne and Shen Song
Abstract: BACKGROUND Few literatures pertain to the 16S ribosomal DNA (16S rDNA) analysis of bacteria contributing to primary and persistent endodontic lesions, with no information available for the Chinese population. As such, we investigated endodontic bacteria associated with primary and persistent endodontic lesions in adult Chinese patients living in Beijing, China using 16S rDNA gene sequencing techniques. METHODS Endodontic microbial samples were obtained from fourteen adult Chinese patients and subjected to DNA extraction. Pllymerase chain reaction (PCR) products were cloned and 100 clones from each generated library were randomly selected. Purified plasmid DNA with 16S rDNA gene inserts was sequenced, and the sequences were searched against GenBank databases using the BLASTN algorithm. Only significant identification with the highest-scored BLAST result and 99% minimum similarity was considered for phylotyping. RESULTS More than 150 taxa were obtained. Primary endodontic infection was mainly associated with Burkholderia cepacia, Actinomyces, Aranicola spp. and Streptococcus sanguinis, whilst Burkholderia cepacia was predominant in the persistent endodontic infections. CONCLUSION There is a difference in the species profile associated with endodontic infections of Chinese patients living in Beijing in comparison to other geographical or ethnic reports.
Keywords: 16S ribosomal DNA; endodontic bacteria; persistent infections; primary infections
Rights: Copyright 2013 Chinese Medical Journal
RMID: 0020125811
DOI: 10.3760/cma.j.issn.0366-6999.20122285
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Appears in Collections:Dentistry publications

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