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|dc.identifier.citation||Molecular and General Genetics, 1989; 218(2):367-370||en|
|dc.description.abstract||Vibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.||en|
|dc.description.statementofresponsibility||Helena M. Ward and Paul A. Manning||en|
|dc.rights||© Springer-Verlag 1989||en|
|dc.subject||Bacteriophage resistance, O-antigen; Conjugation; LPS; P-factor||en|
|dc.title||Mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680||en|
|dc.identifier.orcid||Ward, H. [0000-0002-3831-1205]||en|
|Appears in Collections:||Medical Education Unit publications|
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