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dc.contributor.authorWard, H.en
dc.contributor.authorManning, P.en
dc.identifier.citationMolecular and General Genetics, 1989; 218(2):367-370en
dc.description.abstractVibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.en
dc.description.statementofresponsibilityHelena M. Ward and Paul A. Manningen
dc.publisherSpringer Verlagen
dc.rights© Springer-Verlag 1989en
dc.subjectBacteriophage resistance, O-antigen; Conjugation; LPS; P-factoren
dc.titleMapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680en
dc.typeJournal articleen
pubs.library.collectionMedical Education Unit publicationsen
dc.identifier.orcidWard, H. [0000-0002-3831-1205]en
Appears in Collections:Medical Education Unit publications

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