Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8342
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Type: Journal article
Title: Reduction of ovulation rate in the rat by administration of a neutrophil-depleting monoclonal antibody
Author: Brannstrom, M.
Bonello, N.
Norman, R.
Robertson, S.
Citation: Journal of Reproductive Immunology, 1995; 29(3):265-270
Publisher: Elsevier Biomedical Press
Issue Date: 1995
ISSN: 0165-0378
1872-7603
Abstract: To investigate the effect of systemic neutrophil depletion on ovulation rate, rats were synchronised with eCG and hCG, and concurrently were administered neutrophil-specific, cytotoxic RP-3 monoclonal antibody (mAb), or an irrelevant, class-matched mAb. Neutrophils in the peripheral blood and in the thecal-luteal area of corpora lutea were detected by immunohistochemical analysis with the neutrophil-specific mAb MCA149 and were found to be reduced in number by 70% and 38% respectively following RP-3 treatment compared to the control group. Ovulation rate, as assessed by counting the number of oocytes in the ampullary region of the oviduct 20 h after hCG administration, was found to be reduced by 27% in the neutrophil-depleted rats. This result provides further evidence that neutrophilic granulocytes play an active role in ovulation in the rat.
Keywords: Neutrophils; Animals; Rats; Rats, Sprague-Dawley; Antibodies, Monoclonal; Ovulation; Female
RMID: 0030005226
DOI: 10.1016/0165-0378(95)00941-D
Appears in Collections:Obstetrics and Gynaecology publications

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