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https://hdl.handle.net/2440/8399
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Type: | Journal article |
Title: | Granulocyte-macrophage colony-stimulating factor: presence in human follicular fluid, protein secretion and mRNA expression by ovarian cells |
Author: | Jasper, M. Brannstrom, M. Olofsson, J. Petrucco, O. Mason, H. Robertson, S. Norman, R. |
Citation: | Molecular Human Reproduction, 1996; 2(8):555-562 |
Publisher: | OXFORD UNIV PRESS |
Issue Date: | 1996 |
ISSN: | 1360-9947 1460-2407 |
Statement of Responsibility: | Melinda J. Jasper, Mats Brännström, Jan I. OIofsson, Ossie M. Petrucco, Helen Mason, Sarah A. Robertson and Robert J. Norman |
Abstract: | In recent years it has become evident that a leukocyte-cytokine network contributes to the paracrine regulation of ovarian function. The objectives of this study were to examine the presence of a potent lympho-haemopoietic cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), in tissues and fluids from human ovaries. In a prospective study, follicular fluid and plasma were collected from naturally cycling women and women undergoing hyperstimulation for in-vitro fertilization (IVF). Granulosa-lutein cells were collected at the time of oocyte recovery for IVF and corpora lutea were collected at the time of hysterectomy for non-ovarian reasons. Culture supernatants from ovarian cell and tissue cultures were harvested on completion of a 48 h incubation. Immunoactive GM-CSF was measured by enzyme-linked immunosorbent assay, and was found to be present at statistically significantly higher levels in follicular fluid (8.9 +/- 0.7 pg/ml) and plasma (11.3 +/- 0.8 pg/ml) of women undergoing hyperstimulation compared to follicular fluid (5.3 +/- 0.3 pg/ml) and plasma (7.1 +/- 0.5 pg/ml) from naturally cycling women. Immunoactive GM-CSF was also detected in culture supernatants of granulosa-lutein cells (47.6 pg/10(5) cells), early luteal phase corpora lutea (0.52 pg/microgram DNA) and mid-luteal phase corpora lutea (0.98 pg/microgram DNA). Furthermore, transcripts for GM-CSF, and both the alpha and beta subunits of the GM-CSF receptor, were detected by reverse transcription polymerase chain reaction (RT-PCR) in granulosa-lutein cell culture preparations and corpora lutea collected during the early, mid- and late luteal phase of the menstrual cycle. These results show that GM-CSF is expressed and secreted by cells within the human ovary, and, together with the finding of expression of mRNA for GM-CSF receptor, suggest a role for GM-CSF in the local regulation of ovarian events. |
Keywords: | Ovary Corpus Luteum Luteal Cells Follicular Fluid Granulosa Cells Cells, Cultured Humans Granulocyte-Macrophage Colony-Stimulating Factor Receptors, Granulocyte-Macrophage Colony-Stimulating Factor RNA, Messenger DNA Primers Polymerase Chain Reaction Gene Expression Base Sequence Protein Conformation Adult Female In Vitro Techniques |
Description: | © European Society of Human Reproduction and Embryology 1996 |
DOI: | 10.1093/molehr/2.8.555 |
Published version: | http://dx.doi.org/10.1093/molehr/2.8.555 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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