Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8479
Citations
Scopus Web of ScienceĀ® Altmetric
?
?
Type: Journal article
Title: Effect of 2,4-dinitrophenol on the energy metabolism of cattle embryos produced by in vitro fertilization and culture
Author: Rieger, D.
McGowan, L.
Cox, S.
Pugh, P.
Thompson, J.
Citation: Reproduction Fertility and Development, 2002; 14(6):339-343
Publisher: C S I R O Publishing
Issue Date: 2002
ISSN: 1031-3613
1448-5990
Statement of
Responsibility: 
D. Rieger, L. T. McGowan, S. F. Cox, P. A. Pugh and J. G. Thompson
Abstract: In cattle embryos, the proportion of ATP produced by glycolysis increases following the major activation of the embryonic genome, and development to the blastocyst stage is improved in the presence of 10 microM 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation, from Day 5 to Day 7 of culture. In Experiment 1 of the present study, culture of cattle embryos in the presence of 10 microM DNP from Day 5 to Day 7 stimulated development to the blastocyst stage, but had no significant effects on oxygen, pyruvate or glucose uptake, or on lactate production. In Experiment 2, culture of cattle embryos in the presence of 10 microM DNP from Day 5 to Day 7, stimulated the metabolism of [2-14C]pyruvate (a measure of Krebs cycle activity) on all of Days 5, 6 and 7, and stimulated metabolism of [5-3H]glucose (a measure of glycolysis) on Day 7 only. The results show that 10 microM DNP stimulates oxidative and glycolytic metabolism in Day-5 to Day-7 cattle embryos, but this does not fully explain the observed increase in developmental competence. We propose that partial inhibition or uncoupling of oxidative phosphorylation may reduce the level of intracellular reactive oxygen species production, thereby facilitating development.
Keywords: Blastocyst; Animals; Cattle; Tritium; Carbon Radioisotopes; Pyruvic Acid; 2,4-Dinitrophenol; Glucose; Adenosine Triphosphate; Uncoupling Agents; Fertilization in Vitro; Culture Techniques; Energy Metabolism; Glycolysis; Embryo, Mammalian
RMID: 0020020318
DOI: 10.1071/RD02038
Published version: http://www.publish.csiro.au/nid/44/paper/RD02038.htm
Appears in Collections:Obstetrics and Gynaecology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.