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https://hdl.handle.net/2440/8488
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Type: | Journal article |
Title: | Cellular composition of primary cultures of human granulosa-lutein cells and effects of cytokines |
Author: | Wang, L. Brannstrom, M. Pascoe, V. Norman, R. |
Citation: | Reproduction Fertility and Development, 1995; 7(1):21-26 |
Publisher: | CSIRO |
Issue Date: | 1995 |
ISSN: | 1031-3613 1448-5990 |
Statement of Responsibility: | Ling Jia Wang, Mats Brännström, Vivianne Pascoe and Robert J. Norrnan |
Abstract: | The cellular composition of cells collected from the follicular fluid obtained during the IVF procedure and cultured in vitro was examined, as well as the effects of two cytokines, interleukin-2 (IL-2) and tumour necrosis factor-alpha (TNF alpha), on the proliferation of individual cell types. After 48 h in culture, most of the cells were granulosa-lutein cells exhibiting positive staining against 3-beta-hydroxysteroid dehydrogenase (3 beta-HSD). About one-third of the total cell population stained positive with monoclonal antibodies against specific antigen sites on lymphohaemopoietic cells, including B-lymphocytes, T-lymphocytes, natural killer cells, monocytes and macrophages. During this 48-h period, IL-2 and TNF alpha significantly (P < 0.05) increased the proportion of leukocyte common antigen (LCA)-positive cells and monocytes. At the end of 144 h in culture, although LCA-positive cells and monocytes were still present, there were fewer. The main targets for the proliferative effects of IL-2 and TNF alpha in this culture system during the first 48 h are leukocytes rather than steroid-producing cells. Thus, any observed effects from the addition of cytokines in this system may be due to indirect effects of cytokine-activated leukocytes on granulosa-lutein cells. |
Keywords: | human leukocyte interleukin-2 in vitro fertilization tumour necrosis factor-α. |
Rights: | © CSIRO 1995 |
DOI: | 10.1071/RD9950021 |
Published version: | http://dx.doi.org/10.1071/rd9950021 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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