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|Title:||Uterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice|
|Citation:||Biology of Reproduction, 1992; 46(6):1069-1079|
|Publisher:||Society for the Study of Reproduction|
|Sarah A. Robertson, Graham Mayrhofer, and Robert F. Seamark|
|Abstract:||Cytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23,000 and 23,000-26,000, respectively. Bioassay of luminal fluids and culture supernatants were negative for the cytokines interleukin-1, interleukin-2, interleukin-3, and tumor necrosis factor-alpha. These studies identify murine uterine epithelium as a potent source of the cytokines GM-CSF and IL-6, which we postulate have potentially important functions in pregnancy through actions on target cells in both the uterus and the conceptus.|
|Keywords:||Uterus; Endometrium; Epithelium; Cells, Cultured; Animals; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred CBA; Mice; Mice, Nude; Mice, SCID; Granulocyte-Macrophage Colony-Stimulating Factor; Interleukin-6; Culture Media; Immunohistochemistry; Estrus; Pregnancy; Pregnancy, Animal; Molecular Weight; Female|
|Rights:||Copyright status unknown|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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