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https://hdl.handle.net/2440/85265
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dc.contributor.author | Brannstrom, M. | - |
dc.contributor.author | Norman, R.J. | - |
dc.contributor.author | Seamark, R.F. | - |
dc.contributor.author | Robertson, S.A. | - |
dc.date.issued | 1994 | - |
dc.identifier.citation | Biology of Reproduction, 1994; 50(1):88-94 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.issn | 1529-7268 | - |
dc.identifier.uri | http://hdl.handle.net/2440/85265 | - |
dc.description.abstract | To examine the production of cytokines by the ovary during ovulation, ovaries were obtained from immature rats and from eCG/hCG-primed immature rats at different stages of the ovulatory process (before hCG injection, 10 h after hCG, and 20 h after hCG) and were perfused in vitro for 5 h. Large quantities of interleukin (IL)-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) bioactivity and smaller amounts of tumor necrosis factor-alpha (TNF alpha) and IL-1 bioactivity were found in the perfusate. IL-2 and IL-3 were not detectable in the perfusion media. The GM-CSF content was significantly higher in the perfusate of ovulating ovaries (obtained 10 h after hCG) compared to the earlier stages. Studies on preovulatory ovaries (prior to hCG injection) revealed that GM-CSF release was not influenced by LH, but was markedly increased when recombinant human IL-1 beta (4 ng/ml) was added to the perfusion medium. IL-6 was released in similar amounts from ovaries at all stages. The identity of bioactive GM-CSF was confirmed by neutralization with a specific polyclonal antibody against murine GM-CSF. Size-exclusion chromatography of perfusion medium revealed peaks of GM-CSF and IL-6 bioactivity at approximate molecular masses of 21-23 kDa and 24-25 kDa, respectively. This study demonstrates that the rat ovary produces IL-6, GM-CSF, TNF alpha, and IL-1 prior to and during the ovulatory process and that there are temporal fluctuations in GM-CSF release with a peak in output at ovulation. | - |
dc.description.statementofresponsibility | Mats Brännström, Robert J. Norman, Robert F. Seamark and Sarah A. Robertson | - |
dc.language.iso | en | - |
dc.publisher | Society for the Study of Reproduction | - |
dc.rights | Copyright status unknown | - |
dc.source.uri | http://dx.doi.org/10.1095/biolreprod50.1.88 | - |
dc.subject | Ovary | - |
dc.subject | Animals | - |
dc.subject | Rats | - |
dc.subject | Rats, Sprague-Dawley | - |
dc.subject | Estradiol | - |
dc.subject | Progesterone | - |
dc.subject | Chorionic Gonadotropin | - |
dc.subject | Tumor Necrosis Factor-alpha | - |
dc.subject | Granulocyte-Macrophage Colony-Stimulating Factor | - |
dc.subject | Interleukin-6 | - |
dc.subject | Antibodies | - |
dc.subject | Cytokines | - |
dc.subject | Ovulation | - |
dc.subject | Molecular Weight | - |
dc.subject | Female | - |
dc.title | Rat ovary produces cytokines during ovulation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1095/biolreprod50.1.88 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Norman, R.J. [0000-0002-3118-3896] | - |
dc.identifier.orcid | Robertson, S.A. [0000-0002-9967-0084] | - |
Appears in Collections: | Aurora harvest 7 Obstetrics and Gynaecology publications |
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