Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/86126
Type: Journal article
Title: Comparative virulence of Streptococcus pneumoniae strains with insertion-duplication, point, and deletion mutations in the pneumolysin gene
Author: Berry, A.
Ogunniyi, A.
Miller, D.
Paton, J.
Citation: Infection and Immunity, 1999; 67(2):981-985
Publisher: American Society for Microbiology
Issue Date: 1999
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Anne M. Berry, A. David Ogunniyi, David C. Miller and James C. Paton
Abstract: Pneumolysin is a 471-amino-acid toxin produced byStreptococcus pneumoniae which has both cytolytic and complement activation properties. We have constructed a derivative of the type 2 S. pneumoniae strain D39 in which the portion of the pneumolysin gene encoding amino acids 55 to 437 has been deleted in-frame. The virulence of this strain (ΔPly) was compared with those of wild-type D39, a pneumolysin insertion-duplication mutant (PLN-A), and a derivative (PdT) carrying a toxin gene with three point mutations known to abolish both cytolytic activity and complement activation. PdT was intermediate in virulence between D39 and either PLN-A or ΔPly in a mouse intraperitoneal challenge model. This provides unequivocal evidence that pneumolysin has an additional property that is not abolished by point mutations which reduce cytotoxicity and complement activation to virtually undetectable levels.
Keywords: Animals; Mice, Inbred BALB C; Mice; Streptococcus pneumoniae; Pneumococcal Infections; Bacterial Proteins; Streptolysins; Mutagenesis, Insertional; Virulence; Gene Duplication; Gene Deletion; Mutation; Point Mutation; Genes, Bacterial
Rights: © 1999, American Society for Microbiology
RMID: 0030008989
Published version: http://iai.asm.org/content/67/2/981
Appears in Collections:Molecular and Biomedical Science publications

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