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Type: Journal article
Title: Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells
Author: Zhang, W.
Seki, M.
Furusaki, S.
Middelberg, A.
Citation: Journal of Bioscience and Bioengineering, 1998; 86(1):72-78
Publisher: Elsevier B.V.
Issue Date: 1998
ISSN: 0922-338X
Statement of
Wei Zhang, Minoru Seki, Shintaro Furusaki, Anton P.J. Middelberg
Abstract: Strawberry (Fragaria ananassa cv. Shikinari) cell suspension cultures carried out in shake flasks for 18 days were investigated for cell growth, anthocyanin synthesis and the development of pigmented cells in relation to the uptake of carbohydrate, extracellular PO4, NO3, NH4 and calcium. Cell viability, extracellular anthocyanin content, pH and electric conductivity of the broth were also monitored. The specific growth rate of strawberry cells at exponential phase was 0.27 and 0.28/day based on fresh and dry weight, respectively. Based on the results of kinetic analysis, a model was developed by incorporating governing equations for the ratio of pigmented cells into a Bailey and Nicholson's model. The model satisfactorily described the cell culture.
Keywords: Strawberry; suspension culture; anthocyanin; kinetics; nutrient uptake; mathematical model
Rights: © 1998 Published by Elsevier B.V.
DOI: 10.1016/S0922-338X(98)80037-8
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Appears in Collections:Aurora harvest 6
Chemical Engineering publications

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