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|Title:||Sex specific differences in placental micro RNA expression in pregnancies complicated by asthma|
|Citation:||Reproductive Sciences, 2010 / vol.17, iss.3, pp.319A|
|Conference Name:||57th Annual Meeting of the Society for Gynecologic Investigation (24 Mar 2010 - 27 Mar 2010 : Orlando, FL)|
|Annette Osei-Kumah, Nicolette Hodyl, Julie Owens, Vicki L Clifton|
|Abstract:||BACKGROUND: Micro RNAs (miRs) are non-coding small RNAs and act as important post-transcriptional regulators of gene expression by altering the abundance or translational effi ciency of mRNAs. miR- mediated gene regulation is important for normal physiological and cellular functions and they play key role in developmental processes. Recent evidence suggests important role of miRs in pathological conditions. We have previously identifi ed sex specifi c differences in placental gene expression associated with strategies for optimal growth and fetal survival in the presence of maternal asthma. The current study sought to examine miR expression in the placenta of pregnancies complicated by asthma and in the event of an asthma exacerbation. AIMS/HYPOTHESIS: We hypothesise that post-transcriptional regulation of genes by miRs may play an important role in conferring sexual dimorphism in placental gene expression during development. The aim of the present study was to determine if there are any differences in placental miRs between male and female placentae in the presence of asthma and asthma exacerbation. METHODS: RNA was extracted from male (n=12) and female (n=12) placenta from pregnancies complicated by asthma and miR analysis was conducted. miRGEN target prediction database was used to identify predicted targets for identifi ed miRs. Target genes were then imported into Ingenuity Pathways Analysis (IPA) software to identify functional networks of differentially expressed miRs. RESULTS: Seventy fi ve miRs were differentially expressed between male and female placentae based on a P-value of less than 0.05. Maternal asthma exacerbation was associated with 24 differntially expressed miRs in female placenta compared with males. This included miR-203 and miR-223 which has been associated with chronic inflammatory diseases and cytokine response. Pathway analysis identifi ed networks involved in glucocorticoids receptor signalling, cytokine signalling, apoptosis, cellular growth and differentiation. CONCLUSIONS: There are differentially expressed miRs in male and female placenta in pregnancies complicated by asthma and also in an event of an exacerbation, which target genes involved in cytokine expression and other immune pathways in the placenta. This may be related to the differential gene regulatory mechanisms initiated by males and females in-utero for growth and survival.|
|Rights:||Copyright status unknown|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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