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https://hdl.handle.net/2440/87626
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Type: | Journal article |
Title: | Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae) |
Author: | Waycott, M. Jones, B.L. Van Dijk, J.K. Robson, H.L. Calladine, A. |
Citation: | American Journal of Botany, 2011; 98(4):e81-e83 |
Publisher: | Botanical Society of America |
Issue Date: | 2011 |
ISSN: | 0002-9122 1537-2197 |
Statement of Responsibility: | Michelle Waycott, Brendan L. Jones, Jent Kornelis Van Dijk, Heather L. A. Robson and Ainsley Calladine |
Abstract: | Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations. |
Keywords: | microsatellite; polyploidy; Solanum centrale |
Rights: | © 2011 Botanical Society of America |
DOI: | 10.3732/ajb.1000356 |
Published version: | http://dx.doi.org/10.3732/ajb.1000356 |
Appears in Collections: | Aurora harvest 2 Ecology, Evolution and Landscape Science publications |
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