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https://hdl.handle.net/2440/8811
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Type: | Journal article |
Title: | Human lung mast cells are enriched in the capacity to produce granulocyte-macrophage colony-stimulating factor in response to lgE-dependent stimulation |
Author: | Okayama, Yoshimichi Kobayashi, Hiroyuki Ashman, Leonie Kay Dobashi, Kunio Nakazawa, Tsugio Holgate, Stephan T. Church, Martin K. Mori, Masatomo |
Citation: | European Journal of Immunology,1998; 28(2):708-715 |
Issue Date: | 1998 |
ISSN: | 0014-2980 |
Statement of Responsibility: | Yoshimichi Okayama, Hiroyuki Kobayashi, Leonie K Ashman, Kunio Dobashi, Tsugio Nakazawa, Stephan T. Holgate, Martin K. Church and Masatomo Mori |
Abstract: | By using reverse transcription-PCR, in situ hybridization, enzyme-linked immunosorbent assay and immunocytochemistry, we have studied the production of granulocyte-macrophage colony-stimulating factor (GM-CSF) in human lung mast cells induced by cross-linkage of high-affinity Fcϵ receptors (FcϵRI). We have also confirmed the bioactivity of GM-CSF released from lung mast cells by investigating the effect of the supernatant from lung mast cells activated with anti-IgE on the release of eosinophil cationic protein (ECP) from eosinophils. Mast cells were purified using affinity magnetic selection with monoclonal antibody (mAb) YB5.B8 (93 – 99 % pure). Purified mast cells were precultured with IgE for 16 h before challenge with 1 μg/ml anti-IgE with or without stem cell factor (SCF). Eosinophils were purified by immunomagnetic negative selection (> 98 % pure). The activation of mast cells via FcϵRI enhanced the intensity of the GM-CSF signal within 2 h and the cells produced GM-CSF protein 4 h after the activation. In the absence of recombinant human (rh) SCF, anti-IgE induced a median GM-CSF response of 202 (< 15 ∼ 681) pg/10⁶ mast cells/ 24 h, whereas in the presence of rhSCF the median IgE-dependent GM-CSF release was 356 (152 ∼ 1216) pg/10⁶ mast cells/24 h. This difference was statistically significant (p = 0.0029, n = 12). In contrast, mast cells produced only a small amount of GM-CSF in the absence of anti-IgE. The mast cell supernatant induced ECP release from eosinophils and the release was significantly inhibited by blocking mAb against GM-CSF. These findings indicate that human mast cells are an important cellular source of GM-CSF and as such may contribute to chronic eosinophil-mediated inflammation. |
Keywords: | Human lung mast cell; Eosinophil; Granulocyte-macrophage colony-stimulating factor; Eosinophil cationic protein; IgE-dependent activation |
Rights: | © 1998 WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany |
DOI: | 10.1002/(SICI)1521-4141(199802)28:02<708::AID-IMMU708>3.0.CO;2-A |
Appears in Collections: | Medicine publications |
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