Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/88387
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Fine mapping and chromosome walking towards the Ror1 locus in barley (Hordeum vulgare L.) |
Author: | Acevedo-Garcia, J. Collins, N. Ahmadinejad, N. Ma, L. Houben, A. Bednarek, P. Benjdia, M. Freialdenhoven, A. Altmuller, J. Nurnberg, P. Reinhardt, R. Schulze-Lefert, P. Panstruga, R. |
Citation: | Theoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):2969-2982 |
Publisher: | Springer |
Issue Date: | 2013 |
ISSN: | 0040-5752 1432-2242 |
Statement of Responsibility: | Johanna Acevedo-Garcia, Nicholas C. Collins, Nahal Ahmadinejad, Lu Ma, Andreas Houben, Pawel Bednarek, Mariam Benjdia, Andreas Freialdenhoven, Janine Altmüller, Peter Nürnberg, Richard Reinhardt, Paul Schulze‑Lefert, Ralph Panstruga |
Abstract: | Recessively inherited loss-of-function alleles of the barley (Hordeum vulgare) Mildew resistance locus o (Mlo) gene confer durable broad-spectrum disease resistance against the obligate biotrophic fungal powdery mildew pathogen Blumeria graminis f.sp. hordei. Previous genetic analyses revealed two barley genes, Ror1 and Ror2, that are Required for mlo-specified resistance and basal defence. While Ror2 was cloned and shown to encode a t-SNARE protein (syntaxin), the molecular nature or Ror1 remained elusive. Ror1 was previously mapped to the centromeric region of the long arm of barley chromosome 1H. Here, we narrowed the barley Ror1 interval to 0.18 cM and initiated a chromosome walk using barley yeast artificial chromosome (YAC) clones, next-generation DNA sequencing and fluorescence in situ hybridization. Two non-overlapping YAC contigs containing Ror1 flanking genes were identified. Despite a high degree of synteny observed between barley and the sequenced genomes of the grasses rice (Oryza sativa), Brachypodium distachyon and Sorghum bicolor across the wider chromosomal area, the genes in the YAC contigs showed extensive interspecific rearrangements in orientation and order. Consequently, the position of a Ror1 homolog in these species could not be precisely predicted, nor was a barley gene co-segregating with Ror1 identified. These factors have prevented the molecular identification of the Ror1 gene for the time being. |
Keywords: | Chromosomes, Plant Hordeum In Situ Hybridization, Fluorescence Chromosome Mapping Chromosome Walking Genes, Plant Brachypodium |
Rights: | © Springer-Verlag Berlin Heidelberg 2013 |
DOI: | 10.1007/s00122-013-2186-6 |
Published version: | http://dx.doi.org/10.1007/s00122-013-2186-6 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.